Remodeling of Cytokinin Metabolism at Infection Sites of Colletotrichum graminicola on Maize Leaves

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00381049" target="_blank" >RIV/61389030:_____/12:00381049 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1094/MPMI-01-12-0012-R" target="_blank" >http://dx.doi.org/10.1094/MPMI-01-12-0012-R</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1094/MPMI-01-12-0012-R" target="_blank" >10.1094/MPMI-01-12-0012-R</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Remodeling of Cytokinin Metabolism at Infection Sites of Colletotrichum graminicola on Maize Leaves

Popis výsledku v původním jazyce

When inoculated onto maize leaves at the onset of senescence, the hemibiotroph Colletotrichum graminicola causes green islands that are surrounded by senescing tissue. Taking advantage of green islands as indicators of sites of the establishment of successful infection and of advanced high-performance liquid chromatography tandem mass spectrometry methodology, we analyzed changes in the patterns and levels of cytokinins (CK) at high spatial and analytical resolution. Twenty individual CK were detected in green islands. Levels of cis-zeatin-9-riboside and cis-zeatin-9-riboside-5'-monophosphate increased drastically, whereas that of the most prominent CK, cis-zeatin-O-glucoside, decreased. The fungus likely performed these conversions because corresponding activities were also detected in in vitro cultures amended with CK. We found no evidence that C. graminicola is able to synthesize CK entirely de novo in minimal medium but, after adding dimethylallyl diphosphate, a precursor of CK bio

Název v anglickém jazyce

Remodeling of Cytokinin Metabolism at Infection Sites of Colletotrichum graminicola on Maize Leaves

Popis výsledku anglicky

When inoculated onto maize leaves at the onset of senescence, the hemibiotroph Colletotrichum graminicola causes green islands that are surrounded by senescing tissue. Taking advantage of green islands as indicators of sites of the establishment of successful infection and of advanced high-performance liquid chromatography tandem mass spectrometry methodology, we analyzed changes in the patterns and levels of cytokinins (CK) at high spatial and analytical resolution. Twenty individual CK were detected in green islands. Levels of cis-zeatin-9-riboside and cis-zeatin-9-riboside-5'-monophosphate increased drastically, whereas that of the most prominent CK, cis-zeatin-O-glucoside, decreased. The fungus likely performed these conversions because corresponding activities were also detected in in vitro cultures amended with CK. We found no evidence that C. graminicola is able to synthesize CK entirely de novo in minimal medium but, after adding dimethylallyl diphosphate, a precursor of CK bio

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Plant-Microbe Interactions

ISSN

0894-0282

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1073-1082

Kód UT WoS článku

000306116300006

EID výsledku v databázi Scopus

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