Regulation of tissue differentiation by plant growth regulators on tTCLs of Panax ginseng adventitious roots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00382535" target="_blank" >RIV/61389030:_____/12:00382535 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.indcrop.2011.06.028" target="_blank" >http://dx.doi.org/10.1016/j.indcrop.2011.06.028</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.indcrop.2011.06.028" target="_blank" >10.1016/j.indcrop.2011.06.028</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Regulation of tissue differentiation by plant growth regulators on tTCLs of Panax ginseng adventitious roots

Popis výsledku v původním jazyce

Differentiated tissue in Panax ginseng cultures was found to be very efficacious for saponin production. In order to increase the yield of saponins and preserve culture stability we were testing different plant growth regulators (PGR) and auxin/cytokinincombinations to regulate a level of tissue differentiation. For this purpose we used transverse thin cell layers (tTCLs) of adventitious roots of Panax ginseng. Adventitious roots were cultivated in Shenk and Hildebrand (SH) liquid medium supplemented with IBA (24.6 mu M). Callus formation and root multiplication of adventitious root tTCLs was evaluated after 4 and following 12 weeks of cultivation, respectively, on SH basal medium containing various auxins (3 mg l(-1)) or cytokinins (0.2 or 0.02 mg l(-1)) or their combinations. We found that kinetin (Kin) in combination with auxin benzo[b]selenienyl acetic acid (BSAA), naphthalene acetic acid or indole-3-butric acidis the best for biomass production and following root multiplication.

Název v anglickém jazyce

Regulation of tissue differentiation by plant growth regulators on tTCLs of Panax ginseng adventitious roots

Popis výsledku anglicky

Differentiated tissue in Panax ginseng cultures was found to be very efficacious for saponin production. In order to increase the yield of saponins and preserve culture stability we were testing different plant growth regulators (PGR) and auxin/cytokinincombinations to regulate a level of tissue differentiation. For this purpose we used transverse thin cell layers (tTCLs) of adventitious roots of Panax ginseng. Adventitious roots were cultivated in Shenk and Hildebrand (SH) liquid medium supplemented with IBA (24.6 mu M). Callus formation and root multiplication of adventitious root tTCLs was evaluated after 4 and following 12 weeks of cultivation, respectively, on SH basal medium containing various auxins (3 mg l(-1)) or cytokinins (0.2 or 0.02 mg l(-1)) or their combinations. We found that kinetin (Kin) in combination with auxin benzo[b]selenienyl acetic acid (BSAA), naphthalene acetic acid or indole-3-butric acidis the best for biomass production and following root multiplication.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Industrial Crops and Products

ISSN

0926-6690

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

154-159

Kód UT WoS článku

000296110700021

EID výsledku v databázi Scopus

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