The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00388866" target="_blank" >RIV/61389030:_____/12:00388866 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/12:33142884

Výsledek na webu

<a href="http://dx.doi.org/10.1093/mp/sss045" target="_blank" >http://dx.doi.org/10.1093/mp/sss045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/mp/sss045" target="_blank" >10.1093/mp/sss045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism

Popis výsledku v původním jazyce

A series of reticulated Arabidopsis thaliana mutants were previously described. All mutants show a reticulate leaf pattern, namely green veins on a pale leaf lamina. They have an aberrant mesophyll structure but an intact layer of bundle sheath cells around the veins. Here, we unravel the function of the previously described reticulated EMS-mutant dov1 (differential development of vascular associated cells 1). By positional cloning, we identified the mutated gene, which encodes glutamine phosphoribosylpyrophosphate aminotransferase 2 (ATase2), an enzyme catalyzing the first step of purine nucleotide biosynthesis. dov1 is allelic to the previously characterized cia1-2 mutant that was isolated in a screen for mutants with impaired chloroplast protein import. We show that purine-derived total cytokinins are lowered in dov1 and crosses with phytohormone reporter lines revealed differential reporter activity patterns in dov1. Metabolite profiling unraveled that amino acids that are involve

Název v anglickém jazyce

The Leaf Reticulate Mutant dov1 Is Impaired in the First Step of Purine Metabolism

Popis výsledku anglicky

A series of reticulated Arabidopsis thaliana mutants were previously described. All mutants show a reticulate leaf pattern, namely green veins on a pale leaf lamina. They have an aberrant mesophyll structure but an intact layer of bundle sheath cells around the veins. Here, we unravel the function of the previously described reticulated EMS-mutant dov1 (differential development of vascular associated cells 1). By positional cloning, we identified the mutated gene, which encodes glutamine phosphoribosylpyrophosphate aminotransferase 2 (ATase2), an enzyme catalyzing the first step of purine nucleotide biosynthesis. dov1 is allelic to the previously characterized cia1-2 mutant that was isolated in a screen for mutants with impaired chloroplast protein import. We show that purine-derived total cytokinins are lowered in dov1 and crosses with phytohormone reporter lines revealed differential reporter activity patterns in dov1. Metabolite profiling unraveled that amino acids that are involve

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

<a href="/cs/project/KAN200380801" target="_blank" >KAN200380801: Imunonanotechnologie pro diagnostiku látek hormonální povahy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Plant

ISSN

1674-2052

e-ISSN

—

Svazek periodika

5

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

1227-1241

Kód UT WoS článku

000312573500010

EID výsledku v databázi Scopus

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