Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F13%3A00395044" target="_blank" >RIV/61389030:_____/13:00395044 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11738-012-1126-4" target="_blank" >http://dx.doi.org/10.1007/s11738-012-1126-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11738-012-1126-4" target="_blank" >10.1007/s11738-012-1126-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed

Popis výsledku v původním jazyce

Vernalization-induced flowering is an effect of the epigenetic regulation of gene expression through DNA methylation and histone modifications. Vernalization-mediated silencing of a floral repressor through histone modifications was shown in Arabidopsisthaliana. However, for Brassica napus L., the mechanism underlying vernalization is unclear, and the roles of DNA methylation and histone modifications have not been established. This study revealed the profiles of changes in the DNA methylation state during vernalization (after 14, 35, 56 days) and the subsequent growth in long- or short-day photoperiods (after 2, 7, 14 days) in the winter and spring rapeseed using TLC and MSAP techniques. TLC analysis showed a significant decrease in the amount of 5-methylcytosine (m(5)C) in genomic DNA in both cultivars at the beginning of vernalization, but upon its termination, the winter rape showed a reduced level of m(5)C contrary to a significantly increased level in the spring rape. MSAP analy

Název v anglickém jazyce

Vernalization and photoperiod-related changes in the DNA methylation state in winter and spring rapeseed

Popis výsledku anglicky

Vernalization-induced flowering is an effect of the epigenetic regulation of gene expression through DNA methylation and histone modifications. Vernalization-mediated silencing of a floral repressor through histone modifications was shown in Arabidopsisthaliana. However, for Brassica napus L., the mechanism underlying vernalization is unclear, and the roles of DNA methylation and histone modifications have not been established. This study revealed the profiles of changes in the DNA methylation state during vernalization (after 14, 35, 56 days) and the subsequent growth in long- or short-day photoperiods (after 2, 7, 14 days) in the winter and spring rapeseed using TLC and MSAP techniques. TLC analysis showed a significant decrease in the amount of 5-methylcytosine (m(5)C) in genomic DNA in both cultivars at the beginning of vernalization, but upon its termination, the winter rape showed a reduced level of m(5)C contrary to a significantly increased level in the spring rape. MSAP analy

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

11

Strana od-do

817-827

Kód UT WoS článku

000315035000019

EID výsledku v databázi Scopus

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