In vivo optical detection of pH in microscopic tissue samples of Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F13%3A00424363" target="_blank" >RIV/61389030:_____/13:00424363 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985882:_____/13:00424363

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.msec.2013.07.045" target="_blank" >http://dx.doi.org/10.1016/j.msec.2013.07.045</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.msec.2013.07.045" target="_blank" >10.1016/j.msec.2013.07.045</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo optical detection of pH in microscopic tissue samples of Arabidopsis thaliana

Popis výsledku v původním jazyce

Minimally invasive in vivo measurement of pH inmicroscopic biological samples of ?mor ?l size, e.g. plant cells, tissues and saps, may help to explain complex biological processes. Consequently, techniques to achieve such measurements are a focus of interest for botanists. This paper describes a technique for the in vivo measurement of pH in the range pH 5.0 to pH 7.8 in microscopic plant tissue samples of Arabidopsis thaliana based on a ratiometric fluorescence method using low-loss robust tapered fiber probes. For this purpose tapered fiber probes were prepared and coated with a detection layer containing ion-paired fluorescent pH-transducer 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (c-HPTS). A fluorescence ratiometric approach was employed based on excitation at 415 nm and 450 nm and on the comparison of the fluorescence response at 515 nm. The suitability of tapered fiber probes for local detection of pH between 5.0 and 7.8 was demonstrated. A pH sensitivity of 0.15 pH

Název v anglickém jazyce

In vivo optical detection of pH in microscopic tissue samples of Arabidopsis thaliana

Popis výsledku anglicky

Minimally invasive in vivo measurement of pH inmicroscopic biological samples of ?mor ?l size, e.g. plant cells, tissues and saps, may help to explain complex biological processes. Consequently, techniques to achieve such measurements are a focus of interest for botanists. This paper describes a technique for the in vivo measurement of pH in the range pH 5.0 to pH 7.8 in microscopic plant tissue samples of Arabidopsis thaliana based on a ratiometric fluorescence method using low-loss robust tapered fiber probes. For this purpose tapered fiber probes were prepared and coated with a detection layer containing ion-paired fluorescent pH-transducer 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt (c-HPTS). A fluorescence ratiometric approach was employed based on excitation at 415 nm and 450 nm and on the comparison of the fluorescence response at 515 nm. The suitability of tapered fiber probes for local detection of pH between 5.0 and 7.8 was demonstrated. A pH sensitivity of 0.15 pH

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JA - Elektronika a optoelektronika, elektrotechnika

OECD FORD obor

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Projekt

<a href="/cs/project/GAP102%2F10%2F2139" target="_blank" >GAP102/10/2139: Příprava a vlastnosti křemenných optických skel obsahujících nanočástice dopované prvky vzácných zemin a chromem</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Materials Science & Engineering C-Materials for Biological Applications

ISSN

0928-4931

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

4809-4815

Kód UT WoS článku

000327686900042

EID výsledku v databázi Scopus

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