Determination of Auxin Transport Parameters on the Cellular Level

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00429994" target="_blank" >RIV/61389030:_____/14:00429994 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-1-62703-592-7_22" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-592-7_22</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-62703-592-7_22" target="_blank" >10.1007/978-1-62703-592-7_22</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Auxin Transport Parameters on the Cellular Level

Popis výsledku v původním jazyce

The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin.The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe themethod for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the ac

Název v anglickém jazyce

Determination of Auxin Transport Parameters on the Cellular Level

Popis výsledku anglicky

The accumulation of radioactively labelled compounds in cells is frequently used for the determination of activities of various transport systems located at the plasma membrane, including the system for carrier-mediated transport of plant hormone auxin.The measurements of auxin transport could be performed on the tissue level as well, but for more precise quantitative analysis of activity of individual auxin carriers the model of plant cell cultures represents an invaluable tool. Here, we describe themethod for the determination of the activities of auxin influx and efflux carriers in plant cells grown in a suspension using radiolabelled synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthalene-1-acetic acid (NAA). By making use of specific inhibitors of active auxin influx and efflux, as well as cell lines overexpressing or silencing particular auxin carriers, this method allows the determination of kinetic parameters of auxin flow across the plasma membrane and the ac

Druh

C - Kapitola v odborné knize

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Chemical Genomics

ISBN

978-1-62703-591-0

Počet stran výsledku

13

Strana od-do

241-253

Počet stran knihy

269

Název nakladatele

Humana Press

Místo vydání

Totowa

Kód UT WoS kapitoly

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