N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00431025" target="_blank" >RIV/61389030:_____/14:00431025 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/nph.12519" target="_blank" >http://dx.doi.org/10.1111/nph.12519</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.12519" target="_blank" >10.1111/nph.12519</a>

Jazyk výsledku

angličtina

Název v původním jazyce

N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant

Popis výsledku v původním jazyce

Bacteria communicate with each other in a population density-dependent process known as quorum sensing. N-acyl-homoserine lactones (HSLs) are the autoinducers of Gram-negative bacteria and the best-studied quorum sensing signals so far. HSLs induce various responses in plants, including systemic resistance and root development. Here, we used different methods, including tritium labelling, sensor strain assays and monoclonal antibodies (mAbs), to analyse the uptake and translocation of C8- and C10- homoserine lactones into barley (Hordeum vulgare cv Barke). Both HSLs were already systemically transported into the shoot at 2h after application. HSL uptake could be inhibited by orthovanadate, demonstrating that ABC transporters are involved in the uptake.Root transport occurs predominantly via the central cylinder, which was shown by transport inhibition via KCl application and autoradiography of root cross-sections. Furthermore, a newly established detection method with mAbs allowed the

Název v anglickém jazyce

N-acyl-homoserine lactone uptake and systemic transport in barley rest upon active parts of the plant

Popis výsledku anglicky

Bacteria communicate with each other in a population density-dependent process known as quorum sensing. N-acyl-homoserine lactones (HSLs) are the autoinducers of Gram-negative bacteria and the best-studied quorum sensing signals so far. HSLs induce various responses in plants, including systemic resistance and root development. Here, we used different methods, including tritium labelling, sensor strain assays and monoclonal antibodies (mAbs), to analyse the uptake and translocation of C8- and C10- homoserine lactones into barley (Hordeum vulgare cv Barke). Both HSLs were already systemically transported into the shoot at 2h after application. HSL uptake could be inhibited by orthovanadate, demonstrating that ABC transporters are involved in the uptake.Root transport occurs predominantly via the central cylinder, which was shown by transport inhibition via KCl application and autoradiography of root cross-sections. Furthermore, a newly established detection method with mAbs allowed the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

1469-8137

e-ISSN

—

Svazek periodika

201

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

545-555

Kód UT WoS článku

000328511700019

EID výsledku v databázi Scopus

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