Next-generation sequencing of flow-sorted wheat chromosome 5D reveals lineage-specific translocations and widespread gene duplications

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00441332" target="_blank" >RIV/61389030:_____/14:00441332 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1471-2164-15-1080" target="_blank" >http://dx.doi.org/10.1186/1471-2164-15-1080</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1471-2164-15-1080" target="_blank" >10.1186/1471-2164-15-1080</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Next-generation sequencing of flow-sorted wheat chromosome 5D reveals lineage-specific translocations and widespread gene duplications

Popis výsledku v původním jazyce

Background: The similar to 17 Gb hexaploid bread wheat genome is a high priority and a major technical challenge for genomic studies. In particular, the D sub-genome is relatively lacking in genetic diversity, making it both difficult to map genetically,and a target for introgression of agriculturally useful traits. Elucidating its sequence and structure will therefore facilitate wheat breeding and crop improvement. Results: We generated shotgun sequences from each arm of flow-sorted Triticum aestivumchromosome 5D using 454 FLX Titanium technology, giving 1.34x and 1.61x coverage of the short (5DS) and long (5DL) arms of the chromosome respectively. By a combination of sequence similarity and assembly-based methods, similar to 74% of the sequence reads were classified as repetitive elements, and coding sequence models of 1314 (5DS) and 2975 (5DL) genes were generated. The order of conserved genes in syntenic regions of previously sequenced grass genomes were integrated with physical

Název v anglickém jazyce

Next-generation sequencing of flow-sorted wheat chromosome 5D reveals lineage-specific translocations and widespread gene duplications

Popis výsledku anglicky

Background: The similar to 17 Gb hexaploid bread wheat genome is a high priority and a major technical challenge for genomic studies. In particular, the D sub-genome is relatively lacking in genetic diversity, making it both difficult to map genetically,and a target for introgression of agriculturally useful traits. Elucidating its sequence and structure will therefore facilitate wheat breeding and crop improvement. Results: We generated shotgun sequences from each arm of flow-sorted Triticum aestivumchromosome 5D using 454 FLX Titanium technology, giving 1.34x and 1.61x coverage of the short (5DS) and long (5DL) arms of the chromosome respectively. By a combination of sequence similarity and assembly-based methods, similar to 74% of the sequence reads were classified as repetitive elements, and coding sequence models of 1314 (5DS) and 2975 (5DL) genes were generated. The order of conserved genes in syntenic regions of previously sequenced grass genomes were integrated with physical

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP501%2F12%2FG090" target="_blank" >GBP501/12/G090: Evoluce a funkce komplexních genomů rostlin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

B M C Genomics

ISSN

1471-2164

e-ISSN

—

Svazek periodika

15

Číslo periodika v rámci svazku

DEC 9 2014

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

18

Strana od-do

—

Kód UT WoS článku

000347572200001

EID výsledku v databázi Scopus

—