Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00447821" target="_blank" >RIV/61389030:_____/15:00447821 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/NPLANTS.2015.15" target="_blank" >http://dx.doi.org/10.1038/NPLANTS.2015.15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/NPLANTS.2015.15" target="_blank" >10.1038/NPLANTS.2015.15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1

Popis výsledku v původním jazyce

In Arabidopsis the plasma membrane nitrate transceptor (transporter/receptor) NRT1.1 governs many physiological and developmental responses to nitrate. Alongside facilitating nitrate uptake, NRT1.1 regulates the expression levels of many nitrate assimilation pathway genes, modulates root system architecture, relieves seed dormancy and protects plants from ammonium toxicity. Here, we assess the functional and phenotypic consequences of point mutations in two key residues of NRT1.1 (P492 and T101). We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, andits longer-term downregulation, at high nitrate concentrations. We also show that these mutations have differential effects on genome-wide gene expression. Our findings indicate that NRT1.1 activates four separate signalling mechanisms,

Název v anglickém jazyce

Multiple mechanisms of nitrate sensing by Arabidopsis nitrate transceptor NRT1.1

Popis výsledku anglicky

In Arabidopsis the plasma membrane nitrate transceptor (transporter/receptor) NRT1.1 governs many physiological and developmental responses to nitrate. Alongside facilitating nitrate uptake, NRT1.1 regulates the expression levels of many nitrate assimilation pathway genes, modulates root system architecture, relieves seed dormancy and protects plants from ammonium toxicity. Here, we assess the functional and phenotypic consequences of point mutations in two key residues of NRT1.1 (P492 and T101). We show that the point mutations differentially affect several of the NRT1.1-dependent responses to nitrate, namely the repression of lateral root development at low nitrate concentrations, and the short-term upregulation of the nitrate-uptake gene NRT2.1, andits longer-term downregulation, at high nitrate concentrations. We also show that these mutations have differential effects on genome-wide gene expression. Our findings indicate that NRT1.1 activates four separate signalling mechanisms,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP305%2F11%2F0797" target="_blank" >GAP305/11/0797: Molekulární mechanismy regulující homeostázi rostlinného regulátoru růstu auxinu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Plants

ISSN

2055-026X

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000364391800002

EID výsledku v databázi Scopus

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