Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00451388" target="_blank" >RIV/61389030:_____/15:00451388 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/15:43899808

Výsledek na webu

<a href="http://dx.doi.org/10.3389/fpls.2015.00928" target="_blank" >http://dx.doi.org/10.3389/fpls.2015.00928</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2015.00928" target="_blank" >10.3389/fpls.2015.00928</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

Popis výsledku v původním jazyce

The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 ? NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1

Název v anglickém jazyce

Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

Popis výsledku anglicky

The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 ? NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP501%2F12%2F1942" target="_blank" >GAP501/12/1942: Nespecifická fosfolipasa C: Molekulární, buněčná a funkční charakterizace nového rostlinného enzymu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

—

Svazek periodika

6

Číslo periodika v rámci svazku

NOV 4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000364581700001

EID výsledku v databázi Scopus

2-s2.0-84947424867