Radiation hybrid maps of the D-genome of Aegilops tauschii and their application in sequence assembly of large and complex plant genomes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00451393" target="_blank" >RIV/61389030:_____/15:00451393 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s12864-015-2030-2" target="_blank" >http://dx.doi.org/10.1186/s12864-015-2030-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12864-015-2030-2" target="_blank" >10.1186/s12864-015-2030-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Radiation hybrid maps of the D-genome of Aegilops tauschii and their application in sequence assembly of large and complex plant genomes

Popis výsledku v původním jazyce

The large and complex genome of bread wheat (Triticum aestivum L., similar to 17 Gb) requires high resolution genome maps with saturated marker scaffolds to anchor and orient BAC contigs/sequence scaffolds for whole genome assembly. Radiation hybrid (RH)mapping has proven to be an excellent tool for the development of such maps for it offers much higher and more uniform marker resolution across the length of the chromosome compared to genetic mapping and does not require marker polymorphism per se, asit is based on presence (retention) vs. absence (deletion) marker assay. A total of 609 markers were mapped to 503 unique positions on the seven D-genome chromosomes, with a total map length of 14,706.7 cR. The average distance between any two marker loci was 29.2 cR which corresponds to 2.1 cM or 9.8 Mb. The average mapping resolution across the D-genome was estimated to be 0.34 Mb (Mb/cR) or 0.07 cM (cM/cR). The RH maps showed almost perfect agreement with several published maps with r

Název v anglickém jazyce

Radiation hybrid maps of the D-genome of Aegilops tauschii and their application in sequence assembly of large and complex plant genomes

Popis výsledku anglicky

The large and complex genome of bread wheat (Triticum aestivum L., similar to 17 Gb) requires high resolution genome maps with saturated marker scaffolds to anchor and orient BAC contigs/sequence scaffolds for whole genome assembly. Radiation hybrid (RH)mapping has proven to be an excellent tool for the development of such maps for it offers much higher and more uniform marker resolution across the length of the chromosome compared to genetic mapping and does not require marker polymorphism per se, asit is based on presence (retention) vs. absence (deletion) marker assay. A total of 609 markers were mapped to 503 unique positions on the seven D-genome chromosomes, with a total map length of 14,706.7 cR. The average distance between any two marker loci was 29.2 cR which corresponds to 2.1 cM or 9.8 Mb. The average mapping resolution across the D-genome was estimated to be 0.34 Mb (Mb/cR) or 0.07 cM (cM/cR). The RH maps showed almost perfect agreement with several published maps with r

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP501%2F12%2F2554" target="_blank" >GAP501/12/2554: Fyzická mapa krátkého ramene chromozómu 7D pšenice a její využití pro klonování genu pro rezistenci k mšici zhoubné</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

B M C Genomics

ISSN

1471-2164

e-ISSN

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Svazek periodika

16

Číslo periodika v rámci svazku

OCT 16

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

14

Strana od-do

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Kód UT WoS článku

000362866400002

EID výsledku v databázi Scopus

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