In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00461642" target="_blank" >RIV/61389030:_____/16:00461642 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/16:33160791

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11307-015-0897-6" target="_blank" >http://dx.doi.org/10.1007/s11307-015-0897-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11307-015-0897-6" target="_blank" >10.1007/s11307-015-0897-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores

Popis výsledku v původním jazyce

Some [Ga-68]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores. nnRadiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice. nnCertain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [Zr-89]triacetylfusarinine C (TAFC). All studied Ga-68 and [Zr-89]siderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of [Zr-89]ferrioxamine E (FOXE). nnZr-89 and [Ga-68]siderophores showed analogous in vitro and in vivo behaviour. Tested [Zr-89]siderophores could be applied for longitudinal positron emission tomography (PET) studies of fungal infections and especially TAFC for the development of novel bioconjugates.

Název v anglickém jazyce

In Vitro and In Vivo Comparison of Selected Ga-68 and Zr-89 Labelled Siderophores

Popis výsledku anglicky

Some [Ga-68]siderophores show promise in specific and sensitive imaging of infection. Here, we compare the in vitro and in vivo behaviour of selected Ga-68 and Zr-89 labelled siderophores. nnRadiolabelling was performed in HEPES or sodium acetate buffer systems. Radiochemical purity of labelled siderophores was determined using chromatography. Partition coefficients, in vitro stability and protein binding affinities were determined. Ex vivo biodistribution and animal imaging was studied in mice. nnCertain differences among studied siderophores were observed in labelling efficiency. Protein binding and stability tests showed highest stabilities and lowest protein binding affinities for Ga-68 and [Zr-89]triacetylfusarinine C (TAFC). All studied Ga-68 and [Zr-89]siderophores exhibited a similar biodistribution and pharmacokinetics in mice with the exception of [Zr-89]ferrioxamine E (FOXE). nnZr-89 and [Ga-68]siderophores showed analogous in vitro and in vivo behaviour. Tested [Zr-89]siderophores could be applied for longitudinal positron emission tomography (PET) studies of fungal infections and especially TAFC for the development of novel bioconjugates.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Imaging and Biology

ISSN

1536-1632

e-ISSN

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Svazek periodika

18

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

344-352

Kód UT WoS článku

000376623600005

EID výsledku v databázi Scopus

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