Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00475872" target="_blank" >RIV/61389030:_____/17:00475872 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/17:73584680

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0170715" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0170715</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0170715" target="_blank" >10.1371/journal.pone.0170715</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant

Popis výsledku v původním jazyce

The 78-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 78-1, transcriptomic profiles of the 78-1 malesterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 upregulated and 636 downregulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 78-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 78-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 78-1 anthers. The present study provides insights into the transcriptome of the 78-1 mutant. We identified several genes with altered expression level in 78-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.

Název v anglickém jazyce

Transcriptional regulation of male-sterility in 7B-1 male-sterile tomato mutant

Popis výsledku anglicky

The 78-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it a potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating the male-sterility in 78-1, transcriptomic profiles of the 78-1 malesterile and wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) were identified, including 132 upregulated and 636 downregulated transcripts. Gene ontology (GO) enrichment analysis of DEGs suggested a general impact of the 78-1 mutation on metabolic processes, such as proteolysis and carbohydrate catabolic process. Sixteen candidates with key roles in regulation of anther development were subjected to further analysis using qRT-PCR and in situ hybridization. Cytological studies showed several defects associated with anther development in the 78-1 mutant, including unsynchronized anther maturation, dysfunctional meiosis, arrested microspores, defect in callose degradation and abnormal tapetum development. TUNEL assay showed a defect in programmed cell death (PCD) of tapetal cells in 78-1 anthers. The present study provides insights into the transcriptome of the 78-1 mutant. We identified several genes with altered expression level in 78-1 (including beta-1,3 glucanase, GA2oxs, cystatin, cysteine protease, pectinesterase, TA29, and actin) that could potentially regulate anther developmental processes, such as meiosis, tapetum development, and cell-wall formation/degradation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

19

Strana od-do

—

Kód UT WoS článku

000393712500013

EID výsledku v databázi Scopus

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