Differential responses to isoprenoid, N-6-substituted aromatic cytokinins and indole-3-butyric acid in direct plant regeneration of Eriocephalus africanus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476473" target="_blank" >RIV/61389030:_____/17:00476473 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/17:73584375

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s10725-016-0242-3" target="_blank" >http://dx.doi.org/10.1007/s10725-016-0242-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10725-016-0242-3" target="_blank" >10.1007/s10725-016-0242-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential responses to isoprenoid, N-6-substituted aromatic cytokinins and indole-3-butyric acid in direct plant regeneration of Eriocephalus africanus

Popis výsledku v původním jazyce

Eriocephalus africanus is a medicinal and aromatic plant species that is part of South Africa's remarkable diversity. As a result of illegal and over-harvesting, most plant communities have become unsustainable and as such, effective and efficient conservation strategies have to be implemented. In the present study, an isoprenoid cytokinin (CK): isopentenyladenine (iP) and four aromatic CKs namely benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR) and 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purine (mTTHP) at 1, 5 or 10 mu M were evaluated for in vitro plant regeneration in E. africanus. Different concentrations of indole-3-butyric acid (IBA) were also evaluated for shoot and root organogenesis. The highest number of shoots was produced by mT (1 and 5 mu M) treatment, longest shoots were stimulated by iP (1 mu M) and the highest fresh mass was obtained in BA (5 and 10 mu M), mT (5 and 10 mu M) and mTTHP (5 mu M)-treated plants. During acclimatization, all the in vitro plants obtained from the lowest concentration of CKs survived and 1 mu M iP regenerants recorded a 100% survival rate. For the rooting experiment, more vigorous adventitious roots were observed in the 1 mu M IBA treatment. All IBA treatments had 100% survival rate after 6 weeks of acclimatization. Overall, the concentration and type of plant growth regulators had a remarkable influence on the growth and development of in vitro-derived E. africanus.

Název v anglickém jazyce

Differential responses to isoprenoid, N-6-substituted aromatic cytokinins and indole-3-butyric acid in direct plant regeneration of Eriocephalus africanus

Popis výsledku anglicky

Eriocephalus africanus is a medicinal and aromatic plant species that is part of South Africa's remarkable diversity. As a result of illegal and over-harvesting, most plant communities have become unsustainable and as such, effective and efficient conservation strategies have to be implemented. In the present study, an isoprenoid cytokinin (CK): isopentenyladenine (iP) and four aromatic CKs namely benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR) and 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purine (mTTHP) at 1, 5 or 10 mu M were evaluated for in vitro plant regeneration in E. africanus. Different concentrations of indole-3-butyric acid (IBA) were also evaluated for shoot and root organogenesis. The highest number of shoots was produced by mT (1 and 5 mu M) treatment, longest shoots were stimulated by iP (1 mu M) and the highest fresh mass was obtained in BA (5 and 10 mu M), mT (5 and 10 mu M) and mTTHP (5 mu M)-treated plants. During acclimatization, all the in vitro plants obtained from the lowest concentration of CKs survived and 1 mu M iP regenerants recorded a 100% survival rate. For the rooting experiment, more vigorous adventitious roots were observed in the 1 mu M IBA treatment. All IBA treatments had 100% survival rate after 6 weeks of acclimatization. Overall, the concentration and type of plant growth regulators had a remarkable influence on the growth and development of in vitro-derived E. africanus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/LO1204" target="_blank" >LO1204: Udržitelný rozvoj výzkumu v Centru regionu Haná</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Growth Regulation

ISSN

0167-6903

e-ISSN

—

Svazek periodika

82

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

103-110

Kód UT WoS článku

000399235800010

EID výsledku v databázi Scopus

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