Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476651" target="_blank" >RIV/61389030:_____/17:00476651 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11310/17:10361544
Výsledek na webu
<a href="http://dx.doi.org/10.1080/15226514.2016.1267697" target="_blank" >http://dx.doi.org/10.1080/15226514.2016.1267697</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15226514.2016.1267697" target="_blank" >10.1080/15226514.2016.1267697</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen
Popis výsledku v původním jazyce
Surface waters in urban areas are contaminated by ibuprofen (IBP), a popular and extensively used anti-inflammatory drug. In this study, we investigated the transcriptomic response in Arabidopsis thaliana (L.) Heynh. roots with the aim of revealing genes that are potentially involved in IBP detoxification and elucidating the effect of IBP on plants. IBP upregulated 63 and downregulated 38 transcripts (p-value < 0.1, fold change ≥2) after 2-day exposure to a 5-µM (1.03 mg/L) concentration of IBP under hydroponic conditions. Although the IBP concentration used in the experiment was highly relative to the concentrations found in rivers and wastewater, the number of genes with transcriptional changes was relatively low. The upregulation of cytochrome P450s, glutathione S-transferases, and UDP-glycosyltransferases indicates the occurrence of IBP oxidation in the first phase, followed by conjugation with glutathione and sugar in the second detoxification phase. ABC transporters could be involved in the transport of IBP and its metabolites. The identification of genes potentially involved in IBP detoxification could be useful in an IBP phytoremediation approach.
Název v anglickém jazyce
Transcriptomic response of Arabidopsis thaliana (L.) Heynh. roots to ibuprofen
Popis výsledku anglicky
Surface waters in urban areas are contaminated by ibuprofen (IBP), a popular and extensively used anti-inflammatory drug. In this study, we investigated the transcriptomic response in Arabidopsis thaliana (L.) Heynh. roots with the aim of revealing genes that are potentially involved in IBP detoxification and elucidating the effect of IBP on plants. IBP upregulated 63 and downregulated 38 transcripts (p-value < 0.1, fold change ≥2) after 2-day exposure to a 5-µM (1.03 mg/L) concentration of IBP under hydroponic conditions. Although the IBP concentration used in the experiment was highly relative to the concentrations found in rivers and wastewater, the number of genes with transcriptional changes was relatively low. The upregulation of cytochrome P450s, glutathione S-transferases, and UDP-glycosyltransferases indicates the occurrence of IBP oxidation in the first phase, followed by conjugation with glutathione and sugar in the second detoxification phase. ABC transporters could be involved in the transport of IBP and its metabolites. The identification of genes potentially involved in IBP detoxification could be useful in an IBP phytoremediation approach.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10611 - Plant sciences, botany
Návaznosti výsledku
Projekt
<a href="/cs/project/GA14-22593S" target="_blank" >GA14-22593S: Metabolismus vybraných nesteroidních antiflogistik v rostlinách a jeho dopad na životní prostředí</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International Journal of Phytoremediation
ISSN
1522-6514
e-ISSN
—
Svazek periodika
19
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
6
Strana od-do
695-700
Kód UT WoS článku
000402041800001
EID výsledku v databázi Scopus
2-s2.0-85019615193