Histological and biochemical response of Norway spruce somatic embryos to UV-B irradiation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476855" target="_blank" >RIV/61389030:_____/17:00476855 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00468-017-1547-1" target="_blank" >http://dx.doi.org/10.1007/s00468-017-1547-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00468-017-1547-1" target="_blank" >10.1007/s00468-017-1547-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Histological and biochemical response of Norway spruce somatic embryos to UV-B irradiation

Popis výsledku v původním jazyce

Plants respond to UV-B exposure by synthesizing a broad range of secondary metabolites, including ROS-scavenging antioxidants and UV-B screening phenylpropanoids. Our recent results indicated that the accumulation of higher levels of polyamines in fully developed somatic embryos of Norway spruce may be causally linked to better tolerance of UV-B irradiation. In the present work, we concentrated primarily on changes in phenolics and their localization in irradiated embryos. Somatic embryos, after 14 days of desiccation, were exposed to 6 W m(-2) h(-1) of 312 nm UV-B radiation. The accumulation of higher levels of spermidine and spermine (by about 20%) and total phenolics (about 25%) in these embryos indicates their participation in stress response. Histological localization of polyphenolic compounds was performed under a transmission light microscope. UV-B radiation elicited the accumulation of polyphenolics in the junction zone between root cap and hypocotyl and in the epidermal and subepidermal cells of hypocotyl and cotyledons. The deposition of polyphenolics in intact epidermal cells which were located in the neighbourhood of damaged cells may demonstrate that there is potential for transmission of a signal from injured cells to tissues protected against the direct effects of UV-B irradiation. UV-B irradiation evoked striking polyphenolic accumulation in specialized idioblastic cells localized beneath the epidermis of the somatic embryo hypocotyl and cotyledons. The fluorescence due to flavonoids, detected under confocal laser scanning microscope, increased dramatically after UV-B irradiation in the epidermis of the hypocotyl and cotyledons and in the surface of the root cap of spruce somatic embryos.

Název v anglickém jazyce

Histological and biochemical response of Norway spruce somatic embryos to UV-B irradiation

Popis výsledku anglicky

Plants respond to UV-B exposure by synthesizing a broad range of secondary metabolites, including ROS-scavenging antioxidants and UV-B screening phenylpropanoids. Our recent results indicated that the accumulation of higher levels of polyamines in fully developed somatic embryos of Norway spruce may be causally linked to better tolerance of UV-B irradiation. In the present work, we concentrated primarily on changes in phenolics and their localization in irradiated embryos. Somatic embryos, after 14 days of desiccation, were exposed to 6 W m(-2) h(-1) of 312 nm UV-B radiation. The accumulation of higher levels of spermidine and spermine (by about 20%) and total phenolics (about 25%) in these embryos indicates their participation in stress response. Histological localization of polyphenolic compounds was performed under a transmission light microscope. UV-B radiation elicited the accumulation of polyphenolics in the junction zone between root cap and hypocotyl and in the epidermal and subepidermal cells of hypocotyl and cotyledons. The deposition of polyphenolics in intact epidermal cells which were located in the neighbourhood of damaged cells may demonstrate that there is potential for transmission of a signal from injured cells to tissues protected against the direct effects of UV-B irradiation. UV-B irradiation evoked striking polyphenolic accumulation in specialized idioblastic cells localized beneath the epidermis of the somatic embryo hypocotyl and cotyledons. The fluorescence due to flavonoids, detected under confocal laser scanning microscope, increased dramatically after UV-B irradiation in the epidermis of the hypocotyl and cotyledons and in the surface of the root cap of spruce somatic embryos.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40102 - Forestry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Trees: structure and function

ISSN

0931-1890

e-ISSN

—

Svazek periodika

31

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

15

Strana od-do

1279-1293

Kód UT WoS článku

000405685800015

EID výsledku v databázi Scopus

2-s2.0-85018831070