Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00478868" target="_blank" >RIV/61389030:_____/17:00478868 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.3989/ajbm.2455" target="_blank" >http://dx.doi.org/10.3989/ajbm.2455</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3989/ajbm.2455" target="_blank" >10.3989/ajbm.2455</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH

Popis výsledku v původním jazyce

Festuca subgen. Schedonorus is a group of broad-leaved fescues, which can be divided into two clades: European and Maghrebian. We employed fluorescent in situ hybridization -FISH- with probes specific for 5S and 35S ribosomal DNA and genome size estimation using flow cytometry to shed light on the determination of possible parental genomes of polyploid species of the Maghrebian clade. Our results indicate that octoploid F. arundinacea subsp. atlantigena probably originated from crossing ofthe tetraploids F. arundinacea subsp. fenas -2n = 4x = 28- and F. mairei -2n = 4x = 28- followed by whole genome duplication. However, a large reconstruction of karyotype and genome downsizing has been revealed. Similarly, hexaploid F. arundinacea subsp. corsica presumably resulted from the interspecific hybridization of the diploid F. pratensis and tetraploid F. arundinacea subsp. fenas. Several scenarios on the origin of decaploid F. arundinacea var. letourneuxiana are discussed. This study contributed to our knowledge on the phylogeny of broad-leavedfescues and provided new information on the karyotypes - chromosomenumbers, ploidy levels and numbers and positions of rDNA loci-using FISH and genome size estimations using flow cytometry in selected taxa of this important grass genus.

Název v anglickém jazyce

Cytogenetic relationships within the Maghrebian clade of Festuca subgen. Schedonorus (Poaceae), using flow cytometry and FISH

Popis výsledku anglicky

Festuca subgen. Schedonorus is a group of broad-leaved fescues, which can be divided into two clades: European and Maghrebian. We employed fluorescent in situ hybridization -FISH- with probes specific for 5S and 35S ribosomal DNA and genome size estimation using flow cytometry to shed light on the determination of possible parental genomes of polyploid species of the Maghrebian clade. Our results indicate that octoploid F. arundinacea subsp. atlantigena probably originated from crossing ofthe tetraploids F. arundinacea subsp. fenas -2n = 4x = 28- and F. mairei -2n = 4x = 28- followed by whole genome duplication. However, a large reconstruction of karyotype and genome downsizing has been revealed. Similarly, hexaploid F. arundinacea subsp. corsica presumably resulted from the interspecific hybridization of the diploid F. pratensis and tetraploid F. arundinacea subsp. fenas. Several scenarios on the origin of decaploid F. arundinacea var. letourneuxiana are discussed. This study contributed to our knowledge on the phylogeny of broad-leavedfescues and provided new information on the karyotypes - chromosomenumbers, ploidy levels and numbers and positions of rDNA loci-using FISH and genome size estimations using flow cytometry in selected taxa of this important grass genus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Jardin Botanico de Madrid. Anales

ISSN

0211-1322

e-ISSN

—

Svazek periodika

74

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

ES - Španělské království

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

000408455300006

EID výsledku v databázi Scopus

2-s2.0-85023188672