When simple meets complex: Pollen and the -omics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00485900" target="_blank" >RIV/61389030:_____/17:00485900 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/978-3-319-56645-0_10" target="_blank" >http://dx.doi.org/10.1007/978-3-319-56645-0_10</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-319-56645-0_10" target="_blank" >10.1007/978-3-319-56645-0_10</a>

Jazyk výsledku

angličtina

Název v původním jazyce

When simple meets complex: Pollen and the -omics

Popis výsledku v původním jazyce

Pollen, an extremely reduced bi-cellular or tri-cellular male reproductive structure of flowering plants, serves as a model for numerous studies covering a wide range of developmental and physiological processes. The pollen development and subsequent progamic phase represent two fragile and vital phases of plant ontogenesis, and pollen was among the first singular plant tissues thoroughly characterised at the transcriptomic level. Here we present an overview of high- throughput tools applied in pollen research on numerous plant species. Transcrip- tomics, being the first experimental approach used, has provided and continues providing valuable information about global and specific gene expression and its dynamics. However, the proteome does not fully reflect the transcriptome, namely, because post-transcriptional regulatory levels, especially translation, mRNA storage and protein modifications, are active during male gametophyte development and during progamic phase. Transcriptomics therefore should be complemented by otheromic tools to get more realistic insight, most importantly proteomics and other specialised approaches mapping the involvement of regulatory RNAs and protein post-translational modifications as well as experiments designed to identify the subsets of totalomes like translatome, secretome or allergome.

Název v anglickém jazyce

When simple meets complex: Pollen and the -omics

Popis výsledku anglicky

Pollen, an extremely reduced bi-cellular or tri-cellular male reproductive structure of flowering plants, serves as a model for numerous studies covering a wide range of developmental and physiological processes. The pollen development and subsequent progamic phase represent two fragile and vital phases of plant ontogenesis, and pollen was among the first singular plant tissues thoroughly characterised at the transcriptomic level. Here we present an overview of high- throughput tools applied in pollen research on numerous plant species. Transcrip- tomics, being the first experimental approach used, has provided and continues providing valuable information about global and specific gene expression and its dynamics. However, the proteome does not fully reflect the transcriptome, namely, because post-transcriptional regulatory levels, especially translation, mRNA storage and protein modifications, are active during male gametophyte development and during progamic phase. Transcriptomics therefore should be complemented by otheromic tools to get more realistic insight, most importantly proteomics and other specialised approaches mapping the involvement of regulatory RNAs and protein post-translational modifications as well as experiments designed to identify the subsets of totalomes like translatome, secretome or allergome.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Pollen Tip Growth

ISBN

978-3-319-56644-3

Počet stran výsledku

46

Strana od-do

"2017 s. 247"-"292"

Počet stran knihy

424

Název nakladatele

Springer International Publishing

Místo vydání

Wageningen

Kód UT WoS kapitoly

—