Phosphoprotein enrichment from tobacco mature pollen crude protein extract

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00485921" target="_blank" >RIV/61389030:_____/17:00485921 - isvavai.cz</a>

Výsledek na webu

<a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=28936665" target="_blank" >http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=28936665</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7286-9_21" target="_blank" >10.1007/978-1-4939-7286-9_21</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phosphoprotein enrichment from tobacco mature pollen crude protein extract

Popis výsledku v původním jazyce

Protein phosphorylation was repeatedly shown to be the most dynamic post-translational modification mediated by a huge orchestra of protein kinases and phosphatases. Upon landing on a stigma, pollen grain dehydration and activation are accompanied by changes in protein phosphorylation together with the translation activation of stored mRNAs. To enable studies of the total phosphoproteome, it is usually necessary to apply various enrichment techniques. In this chapter, one of these protocols that worked previously well on tobacco mature pollen is presented in more detail. The method comprises of three basic steps: (1) picking flowers from the flowering tobacco plants (Nicotiana tabacum cv. Samsun), and collection of the shed pollen grains, (2) extraction of total proteins by TCA/acetone, (3) phosphoprotein enrichment by MOAC with aluminum hydroxide matrix. Taken together this protocol describes how to isolate phosphoproteins out of tobacco mature pollen.

Název v anglickém jazyce

Phosphoprotein enrichment from tobacco mature pollen crude protein extract

Popis výsledku anglicky

Protein phosphorylation was repeatedly shown to be the most dynamic post-translational modification mediated by a huge orchestra of protein kinases and phosphatases. Upon landing on a stigma, pollen grain dehydration and activation are accompanied by changes in protein phosphorylation together with the translation activation of stored mRNAs. To enable studies of the total phosphoproteome, it is usually necessary to apply various enrichment techniques. In this chapter, one of these protocols that worked previously well on tobacco mature pollen is presented in more detail. The method comprises of three basic steps: (1) picking flowers from the flowering tobacco plants (Nicotiana tabacum cv. Samsun), and collection of the shed pollen grains, (2) extraction of total proteins by TCA/acetone, (3) phosphoprotein enrichment by MOAC with aluminum hydroxide matrix. Taken together this protocol describes how to isolate phosphoproteins out of tobacco mature pollen.

Druh

C - Kapitola v odborné knize

CEP obor

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OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Plant Germline Development

ISBN

978-1-4939-7285-2

Počet stran výsledku

10

Strana od-do

"Roč. 1669 (2017)"

Počet stran knihy

422

Název nakladatele

Humana Press

Místo vydání

New York

Kód UT WoS kapitoly

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