A Combinatorial Lipid Code Shapes the Electrostatic Landscape of Plant Endomembranes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00492203" target="_blank" >RIV/61389030:_____/18:00492203 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.devcel.2018.04.011" target="_blank" >http://dx.doi.org/10.1016/j.devcel.2018.04.011</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.devcel.2018.04.011" target="_blank" >10.1016/j.devcel.2018.04.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Combinatorial Lipid Code Shapes the Electrostatic Landscape of Plant Endomembranes

Popis výsledku v původním jazyce

Membrane surface charge is critical for the transient, yet specific recruitment of proteins with polybasic regions to certain organelles. In eukaryotes, the plasma membrane (PM) is the most electronegative compartment of the cell, which specifies its identity. As such, membrane electrostatics is a central parameter in signaling, intracellular trafficking, and polarity. Here, we explore which are the lipids that control membrane electrostatics using plants as a model. We show that phosphatidylinositol-4-phosphate (PI4P), phosphatidic acidic (PA), and phosphatidylserine (PS) are separately required to generate the electrostatic signature of the plant PM. In addition, we reveal the existence of an electrostatic territory that is organized as a gradient along the endocytic pathway and is controlled by PS/PI4P combination. Altogether, we propose that combinatorial lipid composition of the cytosolic leaflet of organelles not only defines the electrostatic territory but also distinguishes different functional compartments within this territory by specifying their varying surface charges.

Název v anglickém jazyce

A Combinatorial Lipid Code Shapes the Electrostatic Landscape of Plant Endomembranes

Popis výsledku anglicky

Membrane surface charge is critical for the transient, yet specific recruitment of proteins with polybasic regions to certain organelles. In eukaryotes, the plasma membrane (PM) is the most electronegative compartment of the cell, which specifies its identity. As such, membrane electrostatics is a central parameter in signaling, intracellular trafficking, and polarity. Here, we explore which are the lipids that control membrane electrostatics using plants as a model. We show that phosphatidylinositol-4-phosphate (PI4P), phosphatidic acidic (PA), and phosphatidylserine (PS) are separately required to generate the electrostatic signature of the plant PM. In addition, we reveal the existence of an electrostatic territory that is organized as a gradient along the endocytic pathway and is controlled by PS/PI4P combination. Altogether, we propose that combinatorial lipid composition of the cytosolic leaflet of organelles not only defines the electrostatic territory but also distinguishes different functional compartments within this territory by specifying their varying surface charges.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

<a href="/cs/project/GA17-27477S" target="_blank" >GA17-27477S: Mnohaúrovňová analýza rodiny rostlinných diacylglycerolkinas</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Developmental Cell

ISSN

1534-5807

e-ISSN

—

Svazek periodika

45

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

465-480

Kód UT WoS článku

000435091700009

EID výsledku v databázi Scopus

2-s2.0-85046659170