Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00502554" target="_blank" >RIV/61389030:_____/19:00502554 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/19:80837 RIV/62157124:16370/19:43878252

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.foodchem.2019.01.128" target="_blank" >http://dx.doi.org/10.1016/j.foodchem.2019.01.128</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodchem.2019.01.128" target="_blank" >10.1016/j.foodchem.2019.01.128</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids

Popis výsledku v původním jazyce

Stilbenoids represent a large group of bioactive compounds, which occur in food and medicinal plants. Twenty-five stilbenoids were screened in vitro for their ability to inhibit COX-1, COX-2 and 5-LOX. Piceatannol and pinostilbene showed activity comparable to the zileuton and ibuprofen, respectively. The anti-inflammatory potential of stilbenoids was further evaluated using THP-1 human monocytic leukemia cell line. Tests of the cytotoxicity on the THP-1 and HCT116 cell lines showed very low toxic effects. The tested stilbenoids were evaluated for their ability to attenuate the LPS-stimulated activation of NF-κB/AP-1. Most of the tested substances reduced the activity of NF-κB/AP-1 and later attenuated the expression of TNF-α. The effects of selected stilbenoids were further investigated on inflammatory signaling pathways. Non-prenylated stilbenoids regulated attenuation of NF-ĸB/AP-1 activity upstream by inhibiting the phosphorylation of MAPKs. A docking study used to in silico analyze the tested compounds confirmed their interaction with NF-ĸB, COX-2 and 5-LOX.

Název v anglickém jazyce

Parallel in vitro and in silico investigations into anti-inflammatory effects of non-prenylated stilbenoids

Popis výsledku anglicky

Stilbenoids represent a large group of bioactive compounds, which occur in food and medicinal plants. Twenty-five stilbenoids were screened in vitro for their ability to inhibit COX-1, COX-2 and 5-LOX. Piceatannol and pinostilbene showed activity comparable to the zileuton and ibuprofen, respectively. The anti-inflammatory potential of stilbenoids was further evaluated using THP-1 human monocytic leukemia cell line. Tests of the cytotoxicity on the THP-1 and HCT116 cell lines showed very low toxic effects. The tested stilbenoids were evaluated for their ability to attenuate the LPS-stimulated activation of NF-κB/AP-1. Most of the tested substances reduced the activity of NF-κB/AP-1 and later attenuated the expression of TNF-α. The effects of selected stilbenoids were further investigated on inflammatory signaling pathways. Non-prenylated stilbenoids regulated attenuation of NF-ĸB/AP-1 activity upstream by inhibiting the phosphorylation of MAPKs. A docking study used to in silico analyze the tested compounds confirmed their interaction with NF-ĸB, COX-2 and 5-LOX.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/GA16-07193S" target="_blank" >GA16-07193S: Protizánětlivá aktivita vybraných stilbenů, 2-arylbenzofuranů a jejich metabolitů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Chemistry

ISSN

0308-8146

e-ISSN

—

Svazek periodika

285

Číslo periodika v rámci svazku

JUL 1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

431-440

Kód UT WoS článku

000459823800053

EID výsledku v databázi Scopus

2-s2.0-85061429064