The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants - The functions of 24-epibrassinolide and selenium in the protective mechanisms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00503951" target="_blank" >RIV/61389030:_____/19:00503951 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/19:73593171

Výsledek na webu

<a href="http://doi.org/10.1016/j.plaphy.2019.01.030" target="_blank" >http://doi.org/10.1016/j.plaphy.2019.01.030</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2019.01.030" target="_blank" >10.1016/j.plaphy.2019.01.030</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants - The functions of 24-epibrassinolide and selenium in the protective mechanisms

Popis výsledku v původním jazyce

This study focused on the idea that the toxic effect of zearalenone (ZEA) and the protective actions of the brassinosteroid 24-epibrassinolide (EBR) as well as selenium are dependent on its accumulation in chloroplasts to a high degree. These organelles were isolated from the leaves of oxidative stress-sensitive and stress-tolerant wheat cultivars that had been grown from grains that had been incubated in a solution of ZEA (30 μM), Na 2 SeO 4 (Se, 10 μM), EBR (0.1 μM) or in a mixture of ZEA with Se or EBR. Ultra-high performance liquid chromatography techniques indicated that ZEA was adsorbed in higher amounts in the chloroplasts in the sensitive rather than tolerant cultivar. Although the brassinosteroids and Se were also accumulated in the chloroplasts, higher levels were only found in the tolerant cultivar. The application of EBR increased the homocastasterone content, especially in the chloroplasts of the tolerant plant and after the addition of ZEA. The presence of both protectants caused a decrease in the ZEA content in studied organelles and resulted in diminishing of the oxidative stress (i.e. changes in the activity of the antioxidative enzymes). Moreover, a recovery of photosystem II and decrease in the negative impact of ZEN on Hsp90 transcript accumulation was observed in plants.

Název v anglickém jazyce

The role of chloroplasts in the oxidative stress that is induced by zearalenone in wheat plants - The functions of 24-epibrassinolide and selenium in the protective mechanisms

Popis výsledku anglicky

This study focused on the idea that the toxic effect of zearalenone (ZEA) and the protective actions of the brassinosteroid 24-epibrassinolide (EBR) as well as selenium are dependent on its accumulation in chloroplasts to a high degree. These organelles were isolated from the leaves of oxidative stress-sensitive and stress-tolerant wheat cultivars that had been grown from grains that had been incubated in a solution of ZEA (30 μM), Na 2 SeO 4 (Se, 10 μM), EBR (0.1 μM) or in a mixture of ZEA with Se or EBR. Ultra-high performance liquid chromatography techniques indicated that ZEA was adsorbed in higher amounts in the chloroplasts in the sensitive rather than tolerant cultivar. Although the brassinosteroids and Se were also accumulated in the chloroplasts, higher levels were only found in the tolerant cultivar. The application of EBR increased the homocastasterone content, especially in the chloroplasts of the tolerant plant and after the addition of ZEA. The presence of both protectants caused a decrease in the ZEA content in studied organelles and resulted in diminishing of the oxidative stress (i.e. changes in the activity of the antioxidative enzymes). Moreover, a recovery of photosystem II and decrease in the negative impact of ZEN on Hsp90 transcript accumulation was observed in plants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

—

Svazek periodika

137

Číslo periodika v rámci svazku

APR

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

9

Strana od-do

84-92

Kód UT WoS článku

000462106100008

EID výsledku v databázi Scopus

2-s2.0-85061324819