Endogenous Hypoxia in Lateral Root Primordia Controls Root Architecture by Antagonizing Auxin Signaling in Arabidopsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00504104" target="_blank" >RIV/61389030:_____/19:00504104 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/19:73598568

Výsledek na webu

<a href="http://doi.org/10.1016/j.molp.2019.01.007" target="_blank" >http://doi.org/10.1016/j.molp.2019.01.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molp.2019.01.007" target="_blank" >10.1016/j.molp.2019.01.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Endogenous Hypoxia in Lateral Root Primordia Controls Root Architecture by Antagonizing Auxin Signaling in Arabidopsis

Popis výsledku v původním jazyce

As non-photosynthesizing organs, roots are dependent on diffusion of oxygen from the external environment and, in some instances, from the shoot for their aerobic metabolism. Establishment of hypoxic niches in the developing tissues of plants has been postulated as a consequence of insufficient diffusion of oxygen to satisfy the demands throughout development. Here, we report that such niches are established at specific stages of lateral root primordia development in Arabidopsis thaliana grown under aerobic conditions. Using gain- and loss-of-function mutants, we show that ERF-VII transcription factors, which mediate hypoxic responses, control root architecture by acting in cells with a high level of auxin signaling. ERF-VIIs repress the expression of the auxin-induced genes LBD16, LBD18, and PUCHI, which are essential for lateral root development, by binding to their promoters. Our results support a model in which the establishment of hypoxic niches in the developing lateral root primordia contributes to the shutting down of key auxin-induced genes and regulates the production of lateral roots. This study shows that reduced oxygen availability marks lateral root primordia at late developmental stages in Arabidopsis. Exploiting transgenic plants with altered hypoxia responsiveness, it was established that the local activation of ERF-VII transcription factors by low oxygen antagonizes auxin responses and contributes to terminating the primordium development, thus achieving optimal root system architecture.

Název v anglickém jazyce

Endogenous Hypoxia in Lateral Root Primordia Controls Root Architecture by Antagonizing Auxin Signaling in Arabidopsis

Popis výsledku anglicky

As non-photosynthesizing organs, roots are dependent on diffusion of oxygen from the external environment and, in some instances, from the shoot for their aerobic metabolism. Establishment of hypoxic niches in the developing tissues of plants has been postulated as a consequence of insufficient diffusion of oxygen to satisfy the demands throughout development. Here, we report that such niches are established at specific stages of lateral root primordia development in Arabidopsis thaliana grown under aerobic conditions. Using gain- and loss-of-function mutants, we show that ERF-VII transcription factors, which mediate hypoxic responses, control root architecture by acting in cells with a high level of auxin signaling. ERF-VIIs repress the expression of the auxin-induced genes LBD16, LBD18, and PUCHI, which are essential for lateral root development, by binding to their promoters. Our results support a model in which the establishment of hypoxic niches in the developing lateral root primordia contributes to the shutting down of key auxin-induced genes and regulates the production of lateral roots. This study shows that reduced oxygen availability marks lateral root primordia at late developmental stages in Arabidopsis. Exploiting transgenic plants with altered hypoxia responsiveness, it was established that the local activation of ERF-VII transcription factors by low oxygen antagonizes auxin responses and contributes to terminating the primordium development, thus achieving optimal root system architecture.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Plant

ISSN

1674-2052

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

14

Strana od-do

538-551

Kód UT WoS článku

000462878700010

EID výsledku v databázi Scopus

2-s2.0-85062804372