Strigolactones in an experimental context

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00505026" target="_blank" >RIV/61389030:_____/19:00505026 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/19:10394569

Výsledek na webu

<a href="http://doi.org/10.1007/s10725-019-00502-5" target="_blank" >http://doi.org/10.1007/s10725-019-00502-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10725-019-00502-5" target="_blank" >10.1007/s10725-019-00502-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Strigolactones in an experimental context

Popis výsledku v původním jazyce

Strigolactones (SLs) have become intensively studied phytohormones in recent years. Their role in the regulation of various development processes and stress responses have been established. The biosynthetic and signalling pathways have been gradually described in Arabidopsis, rice, maize, tomato, pea etc. The conserved SL production and function are supported by the identified orthologs of crucial enzymes across the plant kingdom. Using phylogenetic studies orthologs were identified in moss Physcomitrella as well as poplar tree. Extremely low concentrations of endogenous SLs, together with their instability, cause problems with their detection, even if using advanced analytical methods of liquid chromatography-tandem mass spectrometry. To overcome this drawback, synthetic SL analogues were prepared and widely used. The most common is rac-GR24, a racemic mixture of two enantiomers. Recently, it was discovered that rac-GR24 stimulated not only SL but also a parallel karrikin signalling pathway. To ascertain specific SL effects, mutant plants or naturally occurring SLs need to be used. Nonetheless, there is little consensus about SL application which makes it difficult to compare the results of different studies. Moreover, many analogues are being readily synthesised each year. In this review, we aimed to describe the conserved role of MAX/D/RMS genes in SL biosynthesis and signalling with attention to their orthologs in other species. We also tried to emphasise the importance of relevant experimental conditions. It is necessary to take into consideration plant species and age, duration of exposure and SL concentration. Many observed SL effects on plant physiology were found to be concentration dependent.

Název v anglickém jazyce

Strigolactones in an experimental context

Popis výsledku anglicky

Strigolactones (SLs) have become intensively studied phytohormones in recent years. Their role in the regulation of various development processes and stress responses have been established. The biosynthetic and signalling pathways have been gradually described in Arabidopsis, rice, maize, tomato, pea etc. The conserved SL production and function are supported by the identified orthologs of crucial enzymes across the plant kingdom. Using phylogenetic studies orthologs were identified in moss Physcomitrella as well as poplar tree. Extremely low concentrations of endogenous SLs, together with their instability, cause problems with their detection, even if using advanced analytical methods of liquid chromatography-tandem mass spectrometry. To overcome this drawback, synthetic SL analogues were prepared and widely used. The most common is rac-GR24, a racemic mixture of two enantiomers. Recently, it was discovered that rac-GR24 stimulated not only SL but also a parallel karrikin signalling pathway. To ascertain specific SL effects, mutant plants or naturally occurring SLs need to be used. Nonetheless, there is little consensus about SL application which makes it difficult to compare the results of different studies. Moreover, many analogues are being readily synthesised each year. In this review, we aimed to describe the conserved role of MAX/D/RMS genes in SL biosynthesis and signalling with attention to their orthologs in other species. We also tried to emphasise the importance of relevant experimental conditions. It is necessary to take into consideration plant species and age, duration of exposure and SL concentration. Many observed SL effects on plant physiology were found to be concentration dependent.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centrum experimentální biologie rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Growth Regulation

ISSN

0167-6903

e-ISSN

—

Svazek periodika

88

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

16

Strana od-do

113-128

Kód UT WoS článku

000467934900002

EID výsledku v databázi Scopus

2-s2.0-85065289583