A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00508110" target="_blank" >RIV/61389030:_____/19:00508110 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/19:73598464

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.cub.2019.06.083" target="_blank" >http://dx.doi.org/10.1016/j.cub.2019.06.083</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cub.2019.06.083" target="_blank" >10.1016/j.cub.2019.06.083</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants

Popis výsledku v původním jazyce

The diverse forms of today's dominant vascular plant flora are generated by the sustained proliferative activity of sporophyte meristems at plants’ shoot and root tips, a trait known as indeterminacy [1]. Bryophyte sister lineages to the vascular plants lack such indeterminate meristems and have an overall sporophyte form comprising a single small axis that ceases growth in the formation of a reproductive sporangium [1]. Genetic mechanisms regulating indeterminacy are well characterized in flowering plants, involving a feedback loop between class I KNOX genes and cytokinin [2, 3], and class I KNOX expression is a conserved feature of vascular plant meristems [4]. The transition from determinate growth to indeterminacy during evolution was a pre-requisite to vascular plant diversification, but mechanisms enabling the innovation of indeterminacy are unknown [5]. Here, we show that class I KNOX gene activity is necessary and sufficient for axis extension from an intercalary region of determinate moss shoots. As in Arabidopsis, class I KNOX activity can promote cytokinin biosynthesis by an ISOPENTENYL TRANSFERASE gene, PpIPT3. PpIPT3 promotes axis extension, and PpIPT3 and exogenously applied cytokinin can partially compensate for loss of class I KNOX function. By outgroup comparison, the results suggest that a pre-existing KNOX-cytokinin regulatory module was recruited into vascular plant shoot meristems during evolution to promote indeterminacy, thereby enabling the radiation of vascular plant shoot forms.

Název v anglickém jazyce

A KNOX-Cytokinin Regulatory Module Predates the Origin of Indeterminate Vascular Plants

Popis výsledku anglicky

The diverse forms of today's dominant vascular plant flora are generated by the sustained proliferative activity of sporophyte meristems at plants’ shoot and root tips, a trait known as indeterminacy [1]. Bryophyte sister lineages to the vascular plants lack such indeterminate meristems and have an overall sporophyte form comprising a single small axis that ceases growth in the formation of a reproductive sporangium [1]. Genetic mechanisms regulating indeterminacy are well characterized in flowering plants, involving a feedback loop between class I KNOX genes and cytokinin [2, 3], and class I KNOX expression is a conserved feature of vascular plant meristems [4]. The transition from determinate growth to indeterminacy during evolution was a pre-requisite to vascular plant diversification, but mechanisms enabling the innovation of indeterminacy are unknown [5]. Here, we show that class I KNOX gene activity is necessary and sufficient for axis extension from an intercalary region of determinate moss shoots. As in Arabidopsis, class I KNOX activity can promote cytokinin biosynthesis by an ISOPENTENYL TRANSFERASE gene, PpIPT3. PpIPT3 promotes axis extension, and PpIPT3 and exogenously applied cytokinin can partially compensate for loss of class I KNOX function. By outgroup comparison, the results suggest that a pre-existing KNOX-cytokinin regulatory module was recruited into vascular plant shoot meristems during evolution to promote indeterminacy, thereby enabling the radiation of vascular plant shoot forms.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Current Biology

ISSN

0960-9822

e-ISSN

—

Svazek periodika

29

Číslo periodika v rámci svazku

16

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

2743-2750

Kód UT WoS článku

000481587900031

EID výsledku v databázi Scopus

2-s2.0-85070615480