Isotopic labelling for sensitive detection of nanoparticle uptake and translocation in plants from hydroponic medium and soil

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00509269" target="_blank" >RIV/61389030:_____/19:00509269 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1071/EN19064" target="_blank" >http://dx.doi.org/10.1071/EN19064</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1071/EN19064" target="_blank" >10.1071/EN19064</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Isotopic labelling for sensitive detection of nanoparticle uptake and translocation in plants from hydroponic medium and soil

Popis výsledku v původním jazyce

Unintended releases of nanoparticles (NPs) into agricultural soil have recently raised concerns regarding NP accumulation in plants. In this study, specially synthesised isotopically labelled 107Ag-NPs, 65Cu-NPs and 70ZnO-NPs were exposed to three representative plants (Arabidopsis thaliana, Solanum lycopersicum (tomato) and Phragmites australis (common reed)) in hydroponic cultivation and, separately, to tomato plants cultivated in soil at concentrations of 2 mg L-1. Metal concentrations in all samples were analysed by inductively coupled plasma mass spectrometry following acid digestion. The use of isotopically labelled NPs confirmed that elevated levels of metals were from the NP source used for the experiments. Although the highest concentrations of NPs or metals were detected in roots in both hydroponic and soil cultivations, varied levels of translocation to shoots were observed in different plants under hydroponic cultivation. In soil cultivation, where tomato plants were grown to full maturity, low levels of 107Ag (0.38 mg kg-1) with respect to controls were recorded in tomato fruits, 70Zn showed the highest level of translocation to tomato stems (2.72 mg kg-1) and leaves (13.93 mg kg-1). Furthermore, the amounts of NPs retained in the soil (at different depths) after harvesting tomato plants were also determined, the highest concentrations of respective isotopes (1.25 mg kg-1 of 107Ag, 0.79 mg kg-1 of 65Cu, 4.06 mg kg-1 of 70Zn) were found in the top soil layer (∼3 cm). Analysis of NPs exposed to plants in hydroponic medium indicated that the presence of plants increases the dissolution of NPs. Scanning electron microscopy analysis enabled determination of the location of 107Ag-NPs in the roots of tomato plants grown in soil, these NPs were found to accumulate mainly in the cortical cells.

Název v anglickém jazyce

Isotopic labelling for sensitive detection of nanoparticle uptake and translocation in plants from hydroponic medium and soil

Popis výsledku anglicky

Unintended releases of nanoparticles (NPs) into agricultural soil have recently raised concerns regarding NP accumulation in plants. In this study, specially synthesised isotopically labelled 107Ag-NPs, 65Cu-NPs and 70ZnO-NPs were exposed to three representative plants (Arabidopsis thaliana, Solanum lycopersicum (tomato) and Phragmites australis (common reed)) in hydroponic cultivation and, separately, to tomato plants cultivated in soil at concentrations of 2 mg L-1. Metal concentrations in all samples were analysed by inductively coupled plasma mass spectrometry following acid digestion. The use of isotopically labelled NPs confirmed that elevated levels of metals were from the NP source used for the experiments. Although the highest concentrations of NPs or metals were detected in roots in both hydroponic and soil cultivations, varied levels of translocation to shoots were observed in different plants under hydroponic cultivation. In soil cultivation, where tomato plants were grown to full maturity, low levels of 107Ag (0.38 mg kg-1) with respect to controls were recorded in tomato fruits, 70Zn showed the highest level of translocation to tomato stems (2.72 mg kg-1) and leaves (13.93 mg kg-1). Furthermore, the amounts of NPs retained in the soil (at different depths) after harvesting tomato plants were also determined, the highest concentrations of respective isotopes (1.25 mg kg-1 of 107Ag, 0.79 mg kg-1 of 65Cu, 4.06 mg kg-1 of 70Zn) were found in the top soil layer (∼3 cm). Analysis of NPs exposed to plants in hydroponic medium indicated that the presence of plants increases the dissolution of NPs. Scanning electron microscopy analysis enabled determination of the location of 107Ag-NPs in the roots of tomato plants grown in soil, these NPs were found to accumulate mainly in the cortical cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20801 - Environmental biotechnology

Projekt

<a href="/cs/project/8G15003" target="_blank" >8G15003: Příjem nanočástic rostlinami a jeho využití pro dekontaminaci vody a půdy</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Chemistry

ISSN

1448-2517

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

AU - Austrálie

Počet stran výsledku

10

Strana od-do

391-400

Kód UT WoS článku

000486693000003

EID výsledku v databázi Scopus

2-s2.0-85066070588