Seed germination, respiratory processes and phosphatidic acid accumulation in Arabidopsis diacylglycerol kinase knockouts – The effect of brassinosteroid, brassinazole and salinity

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00509274" target="_blank" >RIV/61389030:_____/19:00509274 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.steroids.2019.04.002" target="_blank" >http://dx.doi.org/10.1016/j.steroids.2019.04.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.steroids.2019.04.002" target="_blank" >10.1016/j.steroids.2019.04.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Seed germination, respiratory processes and phosphatidic acid accumulation in Arabidopsis diacylglycerol kinase knockouts – The effect of brassinosteroid, brassinazole and salinity

Popis výsledku v původním jazyce

Using Arabidopsis thaliana wild type (WT)plants and diacylglycerol kinase knockouts (single mutants dgk3, dgk1, dgk6, double mutants dgk3dgk7, dgk5dgk6, dgk1dgk2)we observed that the inhibitor of brassinosteroid (BR)biosynthesis, brassinazole (BRZ), drastically decreased germination of dgk mutants under salt stress, while BRZ co-administration with 24-epibrassinolide (EBL)partially improved germination rates. We also observed a statistically significant decrease in alternative and cytochrome respiratory pathways in response to BRZ treatment under salinity conditions. We showed that production of the lipid second messenger phosphatidic acid (PA)is impaired in dgk mutants in response to EBL treatment and inhibitor of diacylglycerol kinase (DGK)– R59022. This study demonstrates that dgk mutants possess lower germination rates, lower total respiration rates, an alternative respiratory pathway and PA content under optimal and high salinity conditions in response to EBL treatment comparing to WT plants.

Název v anglickém jazyce

Seed germination, respiratory processes and phosphatidic acid accumulation in Arabidopsis diacylglycerol kinase knockouts – The effect of brassinosteroid, brassinazole and salinity

Popis výsledku anglicky

Using Arabidopsis thaliana wild type (WT)plants and diacylglycerol kinase knockouts (single mutants dgk3, dgk1, dgk6, double mutants dgk3dgk7, dgk5dgk6, dgk1dgk2)we observed that the inhibitor of brassinosteroid (BR)biosynthesis, brassinazole (BRZ), drastically decreased germination of dgk mutants under salt stress, while BRZ co-administration with 24-epibrassinolide (EBL)partially improved germination rates. We also observed a statistically significant decrease in alternative and cytochrome respiratory pathways in response to BRZ treatment under salinity conditions. We showed that production of the lipid second messenger phosphatidic acid (PA)is impaired in dgk mutants in response to EBL treatment and inhibitor of diacylglycerol kinase (DGK)– R59022. This study demonstrates that dgk mutants possess lower germination rates, lower total respiration rates, an alternative respiratory pathway and PA content under optimal and high salinity conditions in response to EBL treatment comparing to WT plants.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/EF16_019%2F0000738" target="_blank" >EF16_019/0000738: Centrum experimentální biologie rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Steroids

ISSN

0039-128X

e-ISSN

—

Svazek periodika

147

Číslo periodika v rámci svazku

JUL

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

28-36

Kód UT WoS článku

000487004300006

EID výsledku v databázi Scopus

2-s2.0-85064154965