Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00522617" target="_blank" >RIV/61389030:_____/20:00522617 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/20:73596574

Výsledek na webu

<a href="https://doi.org/10.1016/j.plaphy.2019.11.013" target="_blank" >https://doi.org/10.1016/j.plaphy.2019.11.013</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2019.11.013" target="_blank" >10.1016/j.plaphy.2019.11.013</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap

Popis výsledku v původním jazyce

Hunting cycle of the carnivorous plant Venus flytrap (Dionaea muscipula Ellis) is comprised of mechanism for rapid trap closure followed by slow hermetical sealing and activation of gene expression responsible for digestion of prey and nutrient uptake. In the present study, we focus on the late phase of Venus's flytrap hunting cycle when mechanical stimulation of the prey ceases and is replaced by chemical cues. We used two nitrogen-rich compounds (chitin and protein) in addition to mechanostimulation to investigate the electrical and jasmonate signalling responsible for induction of enzyme activities. Chemical stimulation by BSA protein and chitin did not induce any additional spontaneous action potentials (APs). However, chemical stimulation by protein induced the highest levels of jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) as well as the expression of studied gene encoding a cysteine protease (dionain). Although chitin is probably the first chemical agent which is in direct contact with digestive glands, presence of protein in the secured trap mimics the presence of insect prey best.

Název v anglickém jazyce

Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap

Popis výsledku anglicky

Hunting cycle of the carnivorous plant Venus flytrap (Dionaea muscipula Ellis) is comprised of mechanism for rapid trap closure followed by slow hermetical sealing and activation of gene expression responsible for digestion of prey and nutrient uptake. In the present study, we focus on the late phase of Venus's flytrap hunting cycle when mechanical stimulation of the prey ceases and is replaced by chemical cues. We used two nitrogen-rich compounds (chitin and protein) in addition to mechanostimulation to investigate the electrical and jasmonate signalling responsible for induction of enzyme activities. Chemical stimulation by BSA protein and chitin did not induce any additional spontaneous action potentials (APs). However, chemical stimulation by protein induced the highest levels of jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) as well as the expression of studied gene encoding a cysteine protease (dionain). Although chitin is probably the first chemical agent which is in direct contact with digestive glands, presence of protein in the secured trap mimics the presence of insect prey best.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

—

Svazek periodika

146

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

8

Strana od-do

90-97

Kód UT WoS článku

000510528300008

EID výsledku v databázi Scopus

2-s2.0-85074774325