Molecular mechanisms of plant steroids and study of their interaction with nuclear receptors in prostate cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00524171" target="_blank" >RIV/61389030:_____/20:00524171 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/20:73600125 RIV/61989592:15310/20:73600125

Výsledek na webu

<a href="http://doi.org/10.1016/j.fct.2020.111164" target="_blank" >http://doi.org/10.1016/j.fct.2020.111164</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fct.2020.111164" target="_blank" >10.1016/j.fct.2020.111164</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular mechanisms of plant steroids and study of their interaction with nuclear receptors in prostate cancer cells

Popis výsledku v původním jazyce

Plant hormone brassinosteroids (BRs) have multiple important functions in plants. They have also been found to exhibit anti-tumor, anti-angiogenic and anti-proliferative activity. The experimental part of this article describes the effects of BR biosynthetic precursors on prostate cancer cells. The experiments were performed with LNCaP and DU-145 prostate cancer cell lines. These were cultivated and treated with tested BRs in different concentrations and time intervals. The tested compounds were found to affect cell viability, nuclear receptor expression, cell cycle and apoptosis in the tumor cells. IC50 concentrations were determined based on MTT test and the two most active compounds (cathasterone and 6-oxocampestanol) were used in the next experiments. Cathasterone was the most effective of all tested compounds and effectively inhibited integrity of cell spheres. It was found that both BRs had no significant effect on the cell cycle in LNCaP at IC50 concentration, while in DU-145 a significant block in G0/G1 phase after the BR treatment was observed. The effect of BRs on the nuclear steroid receptors was manifested by changes in their expression and localization. BRs demonstrated their significant effect on prostate cancer cells and the compounds have potential used in anticancer drug research and cancer treatment.

Název v anglickém jazyce

Molecular mechanisms of plant steroids and study of their interaction with nuclear receptors in prostate cancer cells

Popis výsledku anglicky

Plant hormone brassinosteroids (BRs) have multiple important functions in plants. They have also been found to exhibit anti-tumor, anti-angiogenic and anti-proliferative activity. The experimental part of this article describes the effects of BR biosynthetic precursors on prostate cancer cells. The experiments were performed with LNCaP and DU-145 prostate cancer cell lines. These were cultivated and treated with tested BRs in different concentrations and time intervals. The tested compounds were found to affect cell viability, nuclear receptor expression, cell cycle and apoptosis in the tumor cells. IC50 concentrations were determined based on MTT test and the two most active compounds (cathasterone and 6-oxocampestanol) were used in the next experiments. Cathasterone was the most effective of all tested compounds and effectively inhibited integrity of cell spheres. It was found that both BRs had no significant effect on the cell cycle in LNCaP at IC50 concentration, while in DU-145 a significant block in G0/G1 phase after the BR treatment was observed. The effect of BRs on the nuclear steroid receptors was manifested by changes in their expression and localization. BRs demonstrated their significant effect on prostate cancer cells and the compounds have potential used in anticancer drug research and cancer treatment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/GA19-01383S" target="_blank" >GA19-01383S: Modulace steroidních receptorů v nádorových buňkách pomocí brassinosteroidů.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and Chemical Toxicology

ISSN

0278-6915

e-ISSN

—

Svazek periodika

137

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

111164

Kód UT WoS článku

000517659300024

EID výsledku v databázi Scopus

2-s2.0-85078850953