Characterization of lipid peroxidation products formed during ageing of tobacco leaves and effect of manipulated ageing

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531601" target="_blank" >RIV/61389030:_____/20:00531601 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/20:10411857

Výsledek na webu

<a href="http://doi.org/10.1016/j.sajb.2020.05.005" target="_blank" >http://doi.org/10.1016/j.sajb.2020.05.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sajb.2020.05.005" target="_blank" >10.1016/j.sajb.2020.05.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of lipid peroxidation products formed during ageing of tobacco leaves and effect of manipulated ageing

Popis výsledku v původním jazyce

Lipid peroxidation is generally accepted to increase during ageing. We focused on stable lipid peroxidation end products, lipophilic fluorescent pigments (LFP). Their role in ageing in animal/human model has thoroughly been investigated. Changes in characteristics and quantity of LFP during tobacco leaf ageing were studied. We evaluated effect of altered ageing rate on LFP characteristics and accumulation. We employed plants with modulated cytokinins (CK) levels as they are plant hormones controlling development and ageing. Tobacco transgenic plants with senescence-induced CK up-regulation (SAG-ipt plants) displayed rejuvenation of senescent leaves. Slowed ageing was achieved in plants with constitutively down-regulated CK (CKX plants). Both 3D and synchronous fluorescence spectra of LFP indicate more marked differences of fluorophores´ compositions between two different control tobacco cultivars than between control and its transgenic plant. Major fluorophore amount increased with leaf age in both control and CKX plants, in SAG-ipt tobacco its quantity declined in the oldest leaves. The postponed leaf ageing in CKX plants resulted in a diminution of LFP. Higher LFP level in SAG-ipt plants compared with their control leaves of the same age was observed. LFP composition as well as relative abundance of individual LFP might be characteristic for each tobacco cultivar and depend on leaf age.

Název v anglickém jazyce

Characterization of lipid peroxidation products formed during ageing of tobacco leaves and effect of manipulated ageing

Popis výsledku anglicky

Lipid peroxidation is generally accepted to increase during ageing. We focused on stable lipid peroxidation end products, lipophilic fluorescent pigments (LFP). Their role in ageing in animal/human model has thoroughly been investigated. Changes in characteristics and quantity of LFP during tobacco leaf ageing were studied. We evaluated effect of altered ageing rate on LFP characteristics and accumulation. We employed plants with modulated cytokinins (CK) levels as they are plant hormones controlling development and ageing. Tobacco transgenic plants with senescence-induced CK up-regulation (SAG-ipt plants) displayed rejuvenation of senescent leaves. Slowed ageing was achieved in plants with constitutively down-regulated CK (CKX plants). Both 3D and synchronous fluorescence spectra of LFP indicate more marked differences of fluorophores´ compositions between two different control tobacco cultivars than between control and its transgenic plant. Major fluorophore amount increased with leaf age in both control and CKX plants, in SAG-ipt tobacco its quantity declined in the oldest leaves. The postponed leaf ageing in CKX plants resulted in a diminution of LFP. Higher LFP level in SAG-ipt plants compared with their control leaves of the same age was observed. LFP composition as well as relative abundance of individual LFP might be characteristic for each tobacco cultivar and depend on leaf age.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA522%2F03%2F0312" target="_blank" >GA522/03/0312: Studium úlohy volných radikálů v regulaci senescence rostlin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

South African Journal of Botany

ISSN

0254-6299

e-ISSN

—

Svazek periodika

132

Číslo periodika v rámci svazku

Aug

Stát vydavatele periodika

ZA - Jihoafrická republika

Počet stran výsledku

10

Strana od-do

258-267

Kód UT WoS článku

000554662800012

EID výsledku v databázi Scopus

2-s2.0-85086402082