Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00531903" target="_blank" >RIV/61389030:_____/20:00531903 - isvavai.cz</a>

Výsledek na webu

<a href="http://doi.org/10.1007/s00299-020-02544-w" target="_blank" >http://doi.org/10.1007/s00299-020-02544-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00299-020-02544-w" target="_blank" >10.1007/s00299-020-02544-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Popis výsledku v původním jazyce

Key message: This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. nAbstract: Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.

Název v anglickém jazyce

Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors

Popis výsledku anglicky

Key message: This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. nAbstract: Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Reports

ISSN

0721-7714

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

13

Strana od-do

1115-1127

Kód UT WoS článku

000528435000001

EID výsledku v databázi Scopus

2-s2.0-85084142930