The arabidopsis rlck vi_a2 kinase controls seedling and plant growth in parallel with gibberellin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F20%3A00539301" target="_blank" >RIV/61389030:_____/20:00539301 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/20:73604929

Výsledek na webu

<a href="http://doi.org/10.3390/ijms21197266" target="_blank" >http://doi.org/10.3390/ijms21197266</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms21197266" target="_blank" >10.3390/ijms21197266</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The arabidopsis rlck vi_a2 kinase controls seedling and plant growth in parallel with gibberellin

Popis výsledku v původním jazyce

The plant‐specific receptor‐like cytoplasmic kinases (RLCKs) form a large, poorly characterized family. Members of the RLCK VI_A class of dicots have a unique characteristic: their activity is regulated by Rho‐of‐plants (ROP) GTPases. The biological function of one of these kinases was investigated using a T‐DNA insertion mutant and RNA interference. Loss of RLCK VI_A2 function resulted in restricted cell expansion and seedling growth. Although these phenotypes could be rescued by exogenous gibberellin, the mutant did not exhibit lower levels of active gibberellins nor decreased gibberellin sensitivity. Transcriptome analysis confirmed that gibberellin is not the direct target of the kinase, its absence rather affected the metabolism and signalling of other hormones such as auxin. It is hypothesized that gibberellins and the RLCK VI_A2 kinase act in parallel to regulate cell expansion and plant growth. Gene expression studies also indicated that the kinase might have an overlapping role with the transcription factor circuit (PIF4‐BZR1‐ARF6) controlling skotomorphogenesis‐related hypocotyl/cotyledon elongation. Furthermore, the transcriptomic changes revealed that the loss of RLCK VI_A2 function alters cellular processes that are associated with cell membranes, take place at the cell periphery or in the apoplast, and are related to cellular transport and/or cell wall reorganisation.

Název v anglickém jazyce

The arabidopsis rlck vi_a2 kinase controls seedling and plant growth in parallel with gibberellin

Popis výsledku anglicky

The plant‐specific receptor‐like cytoplasmic kinases (RLCKs) form a large, poorly characterized family. Members of the RLCK VI_A class of dicots have a unique characteristic: their activity is regulated by Rho‐of‐plants (ROP) GTPases. The biological function of one of these kinases was investigated using a T‐DNA insertion mutant and RNA interference. Loss of RLCK VI_A2 function resulted in restricted cell expansion and seedling growth. Although these phenotypes could be rescued by exogenous gibberellin, the mutant did not exhibit lower levels of active gibberellins nor decreased gibberellin sensitivity. Transcriptome analysis confirmed that gibberellin is not the direct target of the kinase, its absence rather affected the metabolism and signalling of other hormones such as auxin. It is hypothesized that gibberellins and the RLCK VI_A2 kinase act in parallel to regulate cell expansion and plant growth. Gene expression studies also indicated that the kinase might have an overlapping role with the transcription factor circuit (PIF4‐BZR1‐ARF6) controlling skotomorphogenesis‐related hypocotyl/cotyledon elongation. Furthermore, the transcriptomic changes revealed that the loss of RLCK VI_A2 function alters cellular processes that are associated with cell membranes, take place at the cell periphery or in the apoplast, and are related to cellular transport and/or cell wall reorganisation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

19

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

1-20

Kód UT WoS článku

000586586200001

EID výsledku v databázi Scopus

2-s2.0-85091950234