Metazachlor effect on poplar – Pioneer plant species for riparian buffers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00544188" target="_blank" >RIV/61389030:_____/21:00544188 - isvavai.cz</a>

Výsledek na webu

<a href="http://doi.org/10.1016/j.chemosphere.2021.129711" target="_blank" >http://doi.org/10.1016/j.chemosphere.2021.129711</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2021.129711" target="_blank" >10.1016/j.chemosphere.2021.129711</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metazachlor effect on poplar – Pioneer plant species for riparian buffers

Popis výsledku v původním jazyce

Metazachlor belongs to one of the most used herbicides throughout the world. In order to prevent the contamination of water bodies by such herbicides, the riparian buffers are constructed. The selection of appropriate plant species for this purpose is necessary. In our project, we studied the possibility of grey poplar to uptake and biotransform metazachlor, along with the phytotoxic effect of metazachlor and its metabolites. We used two different models – suspension cultures and poplar regenerants cultivated in vitro. Our results show that the herbicide metazachlor is readily metabolized by both suspension cultures and regenerants to 16 detectable metabolites. The detailed scheme of biotransformation pathway in poplar tissue is presented for the first time. The profile of detected metabolites was approximately the same in poplar cell cultures and regenerants, but the ratio and amounts of particular compounds was significantly different. Generally, the highest concentration (peak area/mg of DW) of all metabolites was present in the roots, the only exception was lactate conjugate (deCl-MZCl-Lact), which accumulated in the cultivation media. Although the plants were not visibly affected by metazachlor or its metabolites, they showed changes in activity of antioxidant enzymes and increased content of phenolic substances, the indicators of stress.

Název v anglickém jazyce

Metazachlor effect on poplar – Pioneer plant species for riparian buffers

Popis výsledku anglicky

Metazachlor belongs to one of the most used herbicides throughout the world. In order to prevent the contamination of water bodies by such herbicides, the riparian buffers are constructed. The selection of appropriate plant species for this purpose is necessary. In our project, we studied the possibility of grey poplar to uptake and biotransform metazachlor, along with the phytotoxic effect of metazachlor and its metabolites. We used two different models – suspension cultures and poplar regenerants cultivated in vitro. Our results show that the herbicide metazachlor is readily metabolized by both suspension cultures and regenerants to 16 detectable metabolites. The detailed scheme of biotransformation pathway in poplar tissue is presented for the first time. The profile of detected metabolites was approximately the same in poplar cell cultures and regenerants, but the ratio and amounts of particular compounds was significantly different. Generally, the highest concentration (peak area/mg of DW) of all metabolites was present in the roots, the only exception was lactate conjugate (deCl-MZCl-Lact), which accumulated in the cultivation media. Although the plants were not visibly affected by metazachlor or its metabolites, they showed changes in activity of antioxidant enzymes and increased content of phenolic substances, the indicators of stress.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosphere

ISSN

0045-6535

e-ISSN

1879-1298

Svazek periodika

274

Číslo periodika v rámci svazku

JUL

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

129711

Kód UT WoS článku

000642944800023

EID výsledku v databázi Scopus

2-s2.0-85100014472