Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and assessment of genetic fidelity of regenerants using ISSR and flow cytometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00545705" target="_blank" >RIV/61389030:_____/21:00545705 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027006:_____/21:10174547 RIV/60460709:41320/21:89228 RIV/60460709:41340/21:89228

Výsledek na webu

<a href="http://doi.org/10.1007/s11240-020-01978-0" target="_blank" >http://doi.org/10.1007/s11240-020-01978-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-020-01978-0" target="_blank" >10.1007/s11240-020-01978-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and assessment of genetic fidelity of regenerants using ISSR and flow cytometry

Popis výsledku v původním jazyce

This study aimed to develop an efficient micropropagation protocol for Disanthus cercidifolius Maxim., an ornamental shrub. Sprouting buds of two genotypes (‘Trubaʼ and ‘PdSʼ) were used as an initial plant material. For the in vitro propagation experiment, the nodal segments were cultured on a MS medium supplemented with either N6-benzyladenine (BA) or zeatin (both at 0.5–3 mg l−1). As a control, MS medium without growth regulators was used. The highest number of shoots per explant (6.95 ± 0.33 in genotype ‘PdSʼ and 7.93 ± 0.41 in genotype ‘Trubaʼ) was achieved on a medium supplemented with 2 mg l−1 BA. Half-strength WPM and half-strength MS medium supplemented with indole-3-butyric acid (IBA) (0.1–1 mg l−1) were tested for in vitro rooting of the shoots. Optimal rooting performance was achieved on a half-strength WPM containing 0.5 mg l−1 IBA in genotype ‘PdSʼ, and 0.1 mg l−1 IBA in genotypes ‘Trubaʼ. The rooted plantlets were transferred ex vitro with 85% survival in genotype ‘PdSʼ and 82.5% in genotype ‘Trubaʼ. Eighteen samples from each genotype were subjected to ISSR analysis and flow cytometry to assess plant genetic fidelity after micropropagation. Fifteen ISSR primers gave rise to monomorphic patterns indicating no detected genetic variation in regenerants. Flow cytometric analysis showed that the ploidy level in all tested in vitro regenerants remained stable. The micropropagation protocol optimized here represents a reliable and efficient method for the large-scale production of plants of the ornamental shrub Disanthus cercidifolius.

Název v anglickém jazyce

Micropropagation of an ornamental shrub Disanthus cercidifolius Maxim. and assessment of genetic fidelity of regenerants using ISSR and flow cytometry

Popis výsledku anglicky

This study aimed to develop an efficient micropropagation protocol for Disanthus cercidifolius Maxim., an ornamental shrub. Sprouting buds of two genotypes (‘Trubaʼ and ‘PdSʼ) were used as an initial plant material. For the in vitro propagation experiment, the nodal segments were cultured on a MS medium supplemented with either N6-benzyladenine (BA) or zeatin (both at 0.5–3 mg l−1). As a control, MS medium without growth regulators was used. The highest number of shoots per explant (6.95 ± 0.33 in genotype ‘PdSʼ and 7.93 ± 0.41 in genotype ‘Trubaʼ) was achieved on a medium supplemented with 2 mg l−1 BA. Half-strength WPM and half-strength MS medium supplemented with indole-3-butyric acid (IBA) (0.1–1 mg l−1) were tested for in vitro rooting of the shoots. Optimal rooting performance was achieved on a half-strength WPM containing 0.5 mg l−1 IBA in genotype ‘PdSʼ, and 0.1 mg l−1 IBA in genotypes ‘Trubaʼ. The rooted plantlets were transferred ex vitro with 85% survival in genotype ‘PdSʼ and 82.5% in genotype ‘Trubaʼ. Eighteen samples from each genotype were subjected to ISSR analysis and flow cytometry to assess plant genetic fidelity after micropropagation. Fifteen ISSR primers gave rise to monomorphic patterns indicating no detected genetic variation in regenerants. Flow cytometric analysis showed that the ploidy level in all tested in vitro regenerants remained stable. The micropropagation protocol optimized here represents a reliable and efficient method for the large-scale production of plants of the ornamental shrub Disanthus cercidifolius.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Svazek periodika

144

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

555-566

Kód UT WoS článku

000604208500003

EID výsledku v databázi Scopus

2-s2.0-85098723513