Insights into the role of transcriptional gene silencing in response to herbicide‐treatments in arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00545881" target="_blank" >RIV/61389030:_____/21:00545881 - isvavai.cz</a>

Výsledek na webu

<a href="http://doi.org/10.3390/ijms22073314" target="_blank" >http://doi.org/10.3390/ijms22073314</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms22073314" target="_blank" >10.3390/ijms22073314</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Insights into the role of transcriptional gene silencing in response to herbicide‐treatments in arabidopsis thaliana

Popis výsledku v původním jazyce

Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether transcriptional gene silencing (TGS) and RNA‐directed DNA Methylation (RdDM) pathways mod-ulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild‐type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4‐D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in RE‐ PRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly increased susceptibility to all applied herbicides, and imazethapyr in particular. Tran-scriptomic analysis of the imazethapyr‐treated wild type and ros1 plants revealed a relation of the herbicide upregulated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid bio-synthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1‐ dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment.

Název v anglickém jazyce

Insights into the role of transcriptional gene silencing in response to herbicide‐treatments in arabidopsis thaliana

Popis výsledku anglicky

Herbicide resistance is broadly recognized as the adaptive evolution of weed populations to the intense selection pressure imposed by the herbicide applications. Here, we tested whether transcriptional gene silencing (TGS) and RNA‐directed DNA Methylation (RdDM) pathways mod-ulate resistance to commonly applied herbicides. Using Arabidopsis thaliana wild‐type plants exposed to sublethal doses of glyphosate, imazethapyr, and 2,4‐D, we found a partial loss of TGS and increased susceptibility to herbicides in six out of 11 tested TGS/RdDM mutants. Mutation in RE‐ PRESSOR OF SILENCING 1 (ROS1), that plays an important role in DNA demethylation, leading to strongly increased susceptibility to all applied herbicides, and imazethapyr in particular. Tran-scriptomic analysis of the imazethapyr‐treated wild type and ros1 plants revealed a relation of the herbicide upregulated genes to chemical stimulus, secondary metabolism, stress condition, flavonoid biosynthesis, and epigenetic processes. Hypersensitivity to imazethapyr of the flavonoid bio-synthesis component TRANSPARENT TESTA 4 (TT4) mutant plants strongly suggests that ROS1‐ dependent accumulation of flavonoids is an important mechanism for herbicide stress response in A. thaliana. In summary, our study shows that herbicide treatment affects transcriptional gene silencing pathways and that misregulation of these pathways makes Arabidopsis plants more sensitive to herbicide treatment.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

1422-0067

Svazek periodika

22

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

3314

Kód UT WoS článku

000638669600001

EID výsledku v databázi Scopus

2-s2.0-85102920035