Quantification of endogenous aromatic cytokinins in Pinus radiata embryonal masses after application of heat stress during initiation of somatic embryogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00549497" target="_blank" >RIV/61389030:_____/21:00549497 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/21:73604444

Výsledek na webu

<a href="http://doi.org/10.1007/s00468-020-02047-x" target="_blank" >http://doi.org/10.1007/s00468-020-02047-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00468-020-02047-x" target="_blank" >10.1007/s00468-020-02047-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of endogenous aromatic cytokinins in Pinus radiata embryonal masses after application of heat stress during initiation of somatic embryogenesis

Popis výsledku v původním jazyce

Key Message: Short-term exposure to high temperatures modulates the aromatic cytokinin profile of radiata pine embryonal masses when measured 8 weeks after initiation. Abstract Recent research has demonstrated that cytokinins could be involved in abiotic stress responses. However, little research has been published about the role of endogenous aromatic cytokinins during these processes. Therefore, in this study, different temperature and incubation periods (23 ºC, 8 weeks, 40 ºC, 4 h, 50 ºC, 30 min, 60 ºC, 5 min) were applied during induction of radiata pine somatic embryogenesis and the aromatic cytokinin content of the resulting embryonal masses was measured. Results indicated that temperature during induction had a significant effect on the profile of most of the aromatic cytokinins detected. All tested higher temperatures than control provoked a decline in some free bases, including N6-benzyladenine, ortho-Topolin and para-Topolin. On the other hand, the values of N6-benzyladenine nucleotides, ribosides and irreversible metabolites (9G), as well as para-Topolin ribosides, increased in embryonal masses originating from 40 ºC treatment. These results suggest that despite not being traditionally considered part of the stress response machinery, aromatic cytokinins might be involved in heat-stress responses during early stages of somatic embryogenesis in radiata pine.

Název v anglickém jazyce

Quantification of endogenous aromatic cytokinins in Pinus radiata embryonal masses after application of heat stress during initiation of somatic embryogenesis

Popis výsledku anglicky

Key Message: Short-term exposure to high temperatures modulates the aromatic cytokinin profile of radiata pine embryonal masses when measured 8 weeks after initiation. Abstract Recent research has demonstrated that cytokinins could be involved in abiotic stress responses. However, little research has been published about the role of endogenous aromatic cytokinins during these processes. Therefore, in this study, different temperature and incubation periods (23 ºC, 8 weeks, 40 ºC, 4 h, 50 ºC, 30 min, 60 ºC, 5 min) were applied during induction of radiata pine somatic embryogenesis and the aromatic cytokinin content of the resulting embryonal masses was measured. Results indicated that temperature during induction had a significant effect on the profile of most of the aromatic cytokinins detected. All tested higher temperatures than control provoked a decline in some free bases, including N6-benzyladenine, ortho-Topolin and para-Topolin. On the other hand, the values of N6-benzyladenine nucleotides, ribosides and irreversible metabolites (9G), as well as para-Topolin ribosides, increased in embryonal masses originating from 40 ºC treatment. These results suggest that despite not being traditionally considered part of the stress response machinery, aromatic cytokinins might be involved in heat-stress responses during early stages of somatic embryogenesis in radiata pine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Trees: structure and function

ISSN

0931-1890

e-ISSN

1432-2285

Svazek periodika

35

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

1075-1080

Kód UT WoS článku

000579323900001

EID výsledku v databázi Scopus

2-s2.0-85092742419