Influence of different cytokinins on the phenolic acids and antioxidant activity of two Brachystelma species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00549509" target="_blank" >RIV/61389030:_____/21:00549509 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/21:73610744

Výsledek na webu

<a href="http://doi.org/10.1007/s11240-021-02038-x" target="_blank" >http://doi.org/10.1007/s11240-021-02038-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11240-021-02038-x" target="_blank" >10.1007/s11240-021-02038-x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Influence of different cytokinins on the phenolic acids and antioxidant activity of two Brachystelma species

Popis výsledku v původním jazyce

The genus Brachystelma morphologically ranges from slender climbers to geophytes that mainly occur in Australia, Southeast Asia and sub-Saharan Africa. These species are well-known for their nutrition and medicinal values. The current study evaluated the effect of cytokinins (CKs) on the phenolic acid content and antioxidant activity of two in vitro-regenerated Brachystelma species. We quantified the phenolic acids (using Ultra-high performance liquid chromatography-tandem mass spectrometry, UHPLC-MS/MS) and antioxidant (Oxygen radical absorbance capacity, ORAC) potential of two (B. pulchellum and B. pygmaeum) species that were successfully micropropagated, with the use of three cytokinins (CKs): benzyladenine (BA), isopentenyladenine (iP) and meta-topolin riboside (mTR). The presence of CKs strongly influenced the concentrations of the 11 phenolic acids (consisting of seven hydroxybenzoic acids and four hydroxycinnamic acids) and ORAC of the in vitro-regenerated B. pulchellum and B. pygmaeum. Generally, the levels of hydroxycinnamic acids were higher than the hydroxybenzoic acids in both Brachystelma species. Ferulic (9-55 mu g/g DW) and sinapic (14-97 mu g/g DW) acids were the major phenolic acids in the micro-propagated plants. In both Brachystelma species, the majority of the CK-regenerated plants had higher ORAC relative to the control. The best antioxidant activity (> 70 mu mol/g Trolox equivalents, TE) was obtained from 25 mu M iP and mTR treatments for B. pulchellum and B. pygmaeum, respectively. Overall, the current study demonstrated the ability of CKs to serve as a suitable tool for the production of valuable therapeutic chemicals in the two Brachystelma species.

Název v anglickém jazyce

Influence of different cytokinins on the phenolic acids and antioxidant activity of two Brachystelma species

Popis výsledku anglicky

The genus Brachystelma morphologically ranges from slender climbers to geophytes that mainly occur in Australia, Southeast Asia and sub-Saharan Africa. These species are well-known for their nutrition and medicinal values. The current study evaluated the effect of cytokinins (CKs) on the phenolic acid content and antioxidant activity of two in vitro-regenerated Brachystelma species. We quantified the phenolic acids (using Ultra-high performance liquid chromatography-tandem mass spectrometry, UHPLC-MS/MS) and antioxidant (Oxygen radical absorbance capacity, ORAC) potential of two (B. pulchellum and B. pygmaeum) species that were successfully micropropagated, with the use of three cytokinins (CKs): benzyladenine (BA), isopentenyladenine (iP) and meta-topolin riboside (mTR). The presence of CKs strongly influenced the concentrations of the 11 phenolic acids (consisting of seven hydroxybenzoic acids and four hydroxycinnamic acids) and ORAC of the in vitro-regenerated B. pulchellum and B. pygmaeum. Generally, the levels of hydroxycinnamic acids were higher than the hydroxybenzoic acids in both Brachystelma species. Ferulic (9-55 mu g/g DW) and sinapic (14-97 mu g/g DW) acids were the major phenolic acids in the micro-propagated plants. In both Brachystelma species, the majority of the CK-regenerated plants had higher ORAC relative to the control. The best antioxidant activity (> 70 mu mol/g Trolox equivalents, TE) was obtained from 25 mu M iP and mTR treatments for B. pulchellum and B. pygmaeum, respectively. Overall, the current study demonstrated the ability of CKs to serve as a suitable tool for the production of valuable therapeutic chemicals in the two Brachystelma species.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EF17_048%2F0007323" target="_blank" >EF17_048/0007323: Rozvoj předaplikačního výzkumu v oblasti nano- a biotechnologií</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell, Tissue and Organ Culture

ISSN

0167-6857

e-ISSN

1573-5044

Svazek periodika

145

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

689-699

Kód UT WoS článku

000622678500001

EID výsledku v databázi Scopus

2-s2.0-85101817171