In‐depth sequence analysis of bread wheat vrn1 genes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00553364" target="_blank" >RIV/61389030:_____/21:00553364 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/21:00554436 RIV/61989592:15310/21:73609382

Výsledek na webu

<a href="http://doi.org/10.3390/ijms222212284" target="_blank" >http://doi.org/10.3390/ijms222212284</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ijms222212284" target="_blank" >10.3390/ijms222212284</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In‐depth sequence analysis of bread wheat vrn1 genes

Popis výsledku v původním jazyce

The VERNALIZATION1 (VRN1) gene encodes a MADS‐box transcription factor and plays an important role in the cold‐induced transition from the vegetative to reproductive stage. Allelic variability of VRN1 homoeologs has been associated with large differences in flowering time. The aim of this study was to investigate the genetic variability of VRN1 homoeologs (VRN‐A1, VRN‐B1 and VRN‐D1). We performed an in‐depth sequence analysis of VRN1 homoeologs in a panel of 105 winter and spring varieties of hexaploid wheat. We describe the novel allele Vrn‐B1f with an 836 bp insertion within intron 1 and show its specific expression pattern associated with reduced heading time. We further provide the complete sequence of the Vrn‐A1b allele, revealing a 177 bp insertion in intron 1, which is transcribed into an alternative splice variant. Copy number variation (CNV) analysis of VRN1 homoeologs showed that VRN‐B1 and VRN‐D1 are present in only one copy. The copy number of recessive vrn‐A1 ranged from one to four, while that of dominant Vrn‐A1 was one or two. Different numbers of Vrn‐A1a copies in the spring cultivars Branisovicka IX/49 and Bastion did not significantly affect heading time. We also report on the deletion of secondary structures (G-quadruplex) in promoter sequences of cultivars with more vrn‐A1 copies.

Název v anglickém jazyce

In‐depth sequence analysis of bread wheat vrn1 genes

Popis výsledku anglicky

The VERNALIZATION1 (VRN1) gene encodes a MADS‐box transcription factor and plays an important role in the cold‐induced transition from the vegetative to reproductive stage. Allelic variability of VRN1 homoeologs has been associated with large differences in flowering time. The aim of this study was to investigate the genetic variability of VRN1 homoeologs (VRN‐A1, VRN‐B1 and VRN‐D1). We performed an in‐depth sequence analysis of VRN1 homoeologs in a panel of 105 winter and spring varieties of hexaploid wheat. We describe the novel allele Vrn‐B1f with an 836 bp insertion within intron 1 and show its specific expression pattern associated with reduced heading time. We further provide the complete sequence of the Vrn‐A1b allele, revealing a 177 bp insertion in intron 1, which is transcribed into an alternative splice variant. Copy number variation (CNV) analysis of VRN1 homoeologs showed that VRN‐B1 and VRN‐D1 are present in only one copy. The copy number of recessive vrn‐A1 ranged from one to four, while that of dominant Vrn‐A1 was one or two. Different numbers of Vrn‐A1a copies in the spring cultivars Branisovicka IX/49 and Bastion did not significantly affect heading time. We also report on the deletion of secondary structures (G-quadruplex) in promoter sequences of cultivars with more vrn‐A1 copies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA19-05445S" target="_blank" >GA19-05445S: Studium molekulárních mechanismů vernalizace u pšenice</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Molecular Sciences

ISSN

1422-0067

e-ISSN

1422-0067

Svazek periodika

22

Číslo periodika v rámci svazku

22

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

12284

Kód UT WoS článku

000725873100001

EID výsledku v databázi Scopus

2-s2.0-85118928059