Anaesthetic diethyl ether impairs long-distance electrical and jasmonate signaling in Arabidopsis thaliana

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F21%3A00553376" target="_blank" >RIV/61389030:_____/21:00553376 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/21:73608740

Výsledek na webu

<a href="http://doi.org/10.1016/j.plaphy.2021.11.019" target="_blank" >http://doi.org/10.1016/j.plaphy.2021.11.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plaphy.2021.11.019" target="_blank" >10.1016/j.plaphy.2021.11.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anaesthetic diethyl ether impairs long-distance electrical and jasmonate signaling in Arabidopsis thaliana

Popis výsledku v původním jazyce

General volatile anaesthetics (GVA) inhibit electrical signal propagation in animal neurons. Although plants do not have neurons, they generate and propagate electrical signals systemically from a local damaged leaf to neighbouring leaves. This systemic electrical signal propagation is mediated by ligand-gated glutamate receptor-like (GLR) channels. Here, we investigated the effect of GVA diethyl ether on the systemic electrical and further downstream responses in Arabidopsis thaliana. We monitored electrical signals, cytoplasmic Ca2+ level ([Ca2+]cyt), ultra-weak photon emission, amino acid contents, phytohormone response as well as gene expression in response to heat wounding during diethyl ether anaesthesia. We found complete suppression of electrical and [Ca2+]cyt signal propagation from damaged leaf to neighbouring systemic leaves upon diethyl ether treatment. Concomitantly, jasmonates (JAs) did not accumulate and expression of JA-responsive genes (AOS, OPR3, JAZ10) was not detected in systemic leaves. However local damaged leaves still showed increased [Ca2+]cyt and accumulated high level of JAs and JA-inducible transcripts. An exogenously added GLR ligand, L-glutamate, was not able to trigger Ca2+ wave in etherized plants indicating that GLRs are targeted by diethyl ether, but not specifically. The fact that GVA inhibit electrical signal propagation not only in animals but also in plants is intriguing. However, the cellular response is completely blocked only in systemic leaves, the local damaged leaf still senses damaging stimuli.

Název v anglickém jazyce

Anaesthetic diethyl ether impairs long-distance electrical and jasmonate signaling in Arabidopsis thaliana

Popis výsledku anglicky

General volatile anaesthetics (GVA) inhibit electrical signal propagation in animal neurons. Although plants do not have neurons, they generate and propagate electrical signals systemically from a local damaged leaf to neighbouring leaves. This systemic electrical signal propagation is mediated by ligand-gated glutamate receptor-like (GLR) channels. Here, we investigated the effect of GVA diethyl ether on the systemic electrical and further downstream responses in Arabidopsis thaliana. We monitored electrical signals, cytoplasmic Ca2+ level ([Ca2+]cyt), ultra-weak photon emission, amino acid contents, phytohormone response as well as gene expression in response to heat wounding during diethyl ether anaesthesia. We found complete suppression of electrical and [Ca2+]cyt signal propagation from damaged leaf to neighbouring systemic leaves upon diethyl ether treatment. Concomitantly, jasmonates (JAs) did not accumulate and expression of JA-responsive genes (AOS, OPR3, JAZ10) was not detected in systemic leaves. However local damaged leaves still showed increased [Ca2+]cyt and accumulated high level of JAs and JA-inducible transcripts. An exogenously added GLR ligand, L-glutamate, was not able to trigger Ca2+ wave in etherized plants indicating that GLRs are targeted by diethyl ether, but not specifically. The fact that GVA inhibit electrical signal propagation not only in animals but also in plants is intriguing. However, the cellular response is completely blocked only in systemic leaves, the local damaged leaf still senses damaging stimuli.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

—

Svazek periodika

169

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

FR - Francouzská republika

Počet stran výsledku

11

Strana od-do

311-321

Kód UT WoS článku

000725594000006

EID výsledku v databázi Scopus

2-s2.0-85119491875