Structure-activity relationships of oomycete elicitins uncover the role of reactive oxygen and nitrogen species in triggering plant defense responses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00561309" target="_blank" >RIV/61389030:_____/22:00561309 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/22:73616568 RIV/00216224:14310/22:00127817

Výsledek na webu

<a href="https://doi.org/10.1016/j.plantsci.2022.111239" target="_blank" >https://doi.org/10.1016/j.plantsci.2022.111239</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2022.111239" target="_blank" >10.1016/j.plantsci.2022.111239</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structure-activity relationships of oomycete elicitins uncover the role of reactive oxygen and nitrogen species in triggering plant defense responses

Popis výsledku v původním jazyce

Elicitins are proteinaceous elicitors that induce the hypersensitive response and plant resistance against diverse phytopathogens. Elicitin recognition by membrane receptors or high-affinity sites activates a variety of fast responses including the production of reactive oxygen species (ROS) and nitric oxide (NO), leading to induction of plant defense genes. Beta-cryptogein (CRY) is a basic β-elicitin secreted by the oomycete Phytophthora cryptogea that shows high necrotic activity in some plant species, whereas infestin 1 (INF1) secreted by the oomycete P. infestans belongs to acidic α-elicitins with a significantly weaker capacity to induce necrosis. We compared several mutated forms of β-CRY and INF1 with a modulated capacity to trigger ROS and NO production, bind plant sterols and induce cell death responses in cell cultures of Nicotiana tabacum L. cv. Xanthi. We evidenced a key role of the lysine residue in position 13 in basic elicitins for their biological activity and enhancement of necrotic effects of acidic INF1 by the replacement of the valine residue in position 84 by larger phenylalanine. Studied elicitins activated in differing intensity signaling pathways of ROS, NO and phytohormones jasmonic acid, ethylene and salicylic acid, known to be involved in triggering of hypersensitive response and establishment of systemic resistance.

Název v anglickém jazyce

Structure-activity relationships of oomycete elicitins uncover the role of reactive oxygen and nitrogen species in triggering plant defense responses

Popis výsledku anglicky

Elicitins are proteinaceous elicitors that induce the hypersensitive response and plant resistance against diverse phytopathogens. Elicitin recognition by membrane receptors or high-affinity sites activates a variety of fast responses including the production of reactive oxygen species (ROS) and nitric oxide (NO), leading to induction of plant defense genes. Beta-cryptogein (CRY) is a basic β-elicitin secreted by the oomycete Phytophthora cryptogea that shows high necrotic activity in some plant species, whereas infestin 1 (INF1) secreted by the oomycete P. infestans belongs to acidic α-elicitins with a significantly weaker capacity to induce necrosis. We compared several mutated forms of β-CRY and INF1 with a modulated capacity to trigger ROS and NO production, bind plant sterols and induce cell death responses in cell cultures of Nicotiana tabacum L. cv. Xanthi. We evidenced a key role of the lysine residue in position 13 in basic elicitins for their biological activity and enhancement of necrotic effects of acidic INF1 by the replacement of the valine residue in position 84 by larger phenylalanine. Studied elicitins activated in differing intensity signaling pathways of ROS, NO and phytohormones jasmonic acid, ethylene and salicylic acid, known to be involved in triggering of hypersensitive response and establishment of systemic resistance.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Science

ISSN

0168-9452

e-ISSN

1873-2259

Svazek periodika

319

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

10

Strana od-do

111239

Kód UT WoS článku

000788740900001

EID výsledku v databázi Scopus

2-s2.0-85126650797