New insights into ribosomal DNA variation in apomictic and sexual Taraxacum (Asteraceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F22%3A00562420" target="_blank" >RIV/61389030:_____/22:00562420 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/22:73613660

Výsledek na webu

<a href="https://doi.org/10.1093/botlinnean/boab094" target="_blank" >https://doi.org/10.1093/botlinnean/boab094</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/botlinnean/boab094" target="_blank" >10.1093/botlinnean/boab094</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New insights into ribosomal DNA variation in apomictic and sexual Taraxacum (Asteraceae)

Popis výsledku v původním jazyce

Apomictic genera have a complex evolutionary history, including reticulate hybridization, polyploidization and variation in reproduction modes. Restrictions of functional meiosis in polyploid apomictic taxa considerably hamper gene conversion, leaving footprints of past hybridization events in their genomes, which are masked by the homogenization of tandemly organized arrays of nuclear ribosomal DNA in sexual species. Dandelions (Taraxacum) have a highly complex reticulate evolutionary history. Detailed knowledge remains hazy, earlier investigations of rDNA sequences have uncovered the complex pattern derived from evolution but without direct evidence for any particular processes. We investigated the position and number of 45S and 5S rDNA loci in 38 Taraxacum taxa (covering different reproduction modes, geographical regions and putative phylogenetic groups) using fluorescent in situ hybridization (FISH) and measured genome size and GC content. The ITS1-5.8S-ITS2 regions of four sexual and five apomictic taxa were sequenced to investigate inter- and intra-individual variation. Most species considerably differ in the chromosome positions of loci and karyotype patterns, but mostly share the same number of studied loci (45S:5S) in a 1:2 ratio per haploid genome (x = 8), with six exceptions (up to a 4:2 ratio). Genome size (2C) varies sixfold and with GC content partly distinguishes major evolutionary groups. Sexual taxa show limited variation in sequenced regions (with two to eight variants), but apomictic taxa vary significantly (with 20-36 variants). Extensive reticulate evolution in Taraxacum and subsequent phenomena such as genome repatterning and non-effective concerted evolution are probably the cause of the dynamic nature of Taraxacum karyotypes and the large variation in genome size and rDNA sequences.

Název v anglickém jazyce

New insights into ribosomal DNA variation in apomictic and sexual Taraxacum (Asteraceae)

Popis výsledku anglicky

Apomictic genera have a complex evolutionary history, including reticulate hybridization, polyploidization and variation in reproduction modes. Restrictions of functional meiosis in polyploid apomictic taxa considerably hamper gene conversion, leaving footprints of past hybridization events in their genomes, which are masked by the homogenization of tandemly organized arrays of nuclear ribosomal DNA in sexual species. Dandelions (Taraxacum) have a highly complex reticulate evolutionary history. Detailed knowledge remains hazy, earlier investigations of rDNA sequences have uncovered the complex pattern derived from evolution but without direct evidence for any particular processes. We investigated the position and number of 45S and 5S rDNA loci in 38 Taraxacum taxa (covering different reproduction modes, geographical regions and putative phylogenetic groups) using fluorescent in situ hybridization (FISH) and measured genome size and GC content. The ITS1-5.8S-ITS2 regions of four sexual and five apomictic taxa were sequenced to investigate inter- and intra-individual variation. Most species considerably differ in the chromosome positions of loci and karyotype patterns, but mostly share the same number of studied loci (45S:5S) in a 1:2 ratio per haploid genome (x = 8), with six exceptions (up to a 4:2 ratio). Genome size (2C) varies sixfold and with GC content partly distinguishes major evolutionary groups. Sexual taxa show limited variation in sequenced regions (with two to eight variants), but apomictic taxa vary significantly (with 20-36 variants). Extensive reticulate evolution in Taraxacum and subsequent phenomena such as genome repatterning and non-effective concerted evolution are probably the cause of the dynamic nature of Taraxacum karyotypes and the large variation in genome size and rDNA sequences.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/LO1204" target="_blank" >LO1204: Udržitelný rozvoj výzkumu v Centru regionu Haná</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Botanical Journal of the Linnean Society

ISSN

0024-4074

e-ISSN

1095-8339

Svazek periodika

199

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

26

Strana od-do

790-815

Kód UT WoS článku

000756758600001

EID výsledku v databázi Scopus

2-s2.0-85136228501