An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00575377" target="_blank" >RIV/61389030:_____/23:00575377 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1038/s41588-023-01401-2" target="_blank" >https://doi.org/10.1038/s41588-023-01401-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41588-023-01401-2" target="_blank" >10.1038/s41588-023-01401-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

Popis výsledku v původním jazyce

The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with disease-resistance genes 1. Here we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene Lr9, which was introduced into bread wheat from the wild grass species Aegilops umbellulata 2. We established that Lr9 encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor enabled us to assemble the ~28.4-Mb Lr9 translocation and to identify the translocation breakpoint. We likewise cloned Lr58, which was reportedly introgressed from Aegilops triuncialis 3, but has an identical coding sequence compared to Lr9. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.

Název v anglickém jazyce

An unusual tandem kinase fusion protein confers leaf rust resistance in wheat

Popis výsledku anglicky

The introgression of chromosome segments from wild relatives is an established strategy to enrich crop germplasm with disease-resistance genes 1. Here we use mutagenesis and transcriptome sequencing to clone the leaf rust resistance gene Lr9, which was introduced into bread wheat from the wild grass species Aegilops umbellulata 2. We established that Lr9 encodes an unusual tandem kinase fusion protein. Long-read sequencing of a wheat Lr9 introgression line and the putative Ae. umbellulata Lr9 donor enabled us to assemble the ~28.4-Mb Lr9 translocation and to identify the translocation breakpoint. We likewise cloned Lr58, which was reportedly introgressed from Aegilops triuncialis 3, but has an identical coding sequence compared to Lr9. Cytogenetic and haplotype analyses corroborate that the two genes originate from the same translocation event. Our work sheds light on the emerging role of kinase fusion proteins in wheat disease resistance, expanding the repertoire of disease-resistance genes for breeding.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Rostliny jako prostředek udržitelného globálního rozvoje</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Genetics

ISSN

1061-4036

e-ISSN

1546-1718

Svazek periodika

55

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

914-920

Kód UT WoS článku

000993686600001

EID výsledku v databázi Scopus

2-s2.0-85160034677