Molecular Characterization of Mitogenome of Cacopsylla picta and Cacopsylla melanoneura, Two Vector Species of ‘Candidatus Phytoplasma mali’

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00576197" target="_blank" >RIV/61389030:_____/23:00576197 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/23:00132162 RIV/61989592:15310/23:73620373 RIV/25271121:_____/23:N0000135

Výsledek na webu

<a href="https://doi.org/10.3390/agronomy13092210" target="_blank" >https://doi.org/10.3390/agronomy13092210</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy13092210" target="_blank" >10.3390/agronomy13092210</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Characterization of Mitogenome of Cacopsylla picta and Cacopsylla melanoneura, Two Vector Species of ‘Candidatus Phytoplasma mali’

Popis výsledku v původním jazyce

The mitochondrial genomes of two vector psyllids of the ‘Candidatus Phytoplasma mali’, Cacopsylla picta and C. melanoneura, were sequenced using high-throughput sequencing on the Illumina platform. The main objective of the study was to describe their mitogenome and characterize their genetic variability and the potential changes in the context of the observed global warming. The four complete sequences for C. picta, 14,801 bp and 14,802 bp in length, two complete and one partial sequence for C. melanoneura, ranging from 14,879 bp to 14,881 bp in length, were obtained for the first time for these European apple psyllids. The detected intraspecies mtDNA identity was highly similar (99.85–99.98%), the identity’s similarity with other Cacopsylla species varied between 79.79 and 86.64%. The mitogenomes showed a typical mitochondrial DNA structure with 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes, the presence of CGGA motif in the ND1-trnS2 junction was detected in both species. Phylogenetic analysis placed both species in close relationship with C. burckhardti within the Cacopsylla clade-I O group. The analysis of complete mitogenomes and of partial COI sequences of fifty-two Cacopsylla individuals showed a high homogeneity of genotypes over 15 years and among the different localities in the Czech Republic.

Název v anglickém jazyce

Molecular Characterization of Mitogenome of Cacopsylla picta and Cacopsylla melanoneura, Two Vector Species of ‘Candidatus Phytoplasma mali’

Popis výsledku anglicky

The mitochondrial genomes of two vector psyllids of the ‘Candidatus Phytoplasma mali’, Cacopsylla picta and C. melanoneura, were sequenced using high-throughput sequencing on the Illumina platform. The main objective of the study was to describe their mitogenome and characterize their genetic variability and the potential changes in the context of the observed global warming. The four complete sequences for C. picta, 14,801 bp and 14,802 bp in length, two complete and one partial sequence for C. melanoneura, ranging from 14,879 bp to 14,881 bp in length, were obtained for the first time for these European apple psyllids. The detected intraspecies mtDNA identity was highly similar (99.85–99.98%), the identity’s similarity with other Cacopsylla species varied between 79.79 and 86.64%. The mitogenomes showed a typical mitochondrial DNA structure with 13 protein-coding genes, 2 rRNA genes and 22 tRNA genes, the presence of CGGA motif in the ND1-trnS2 junction was detected in both species. Phylogenetic analysis placed both species in close relationship with C. burckhardti within the Cacopsylla clade-I O group. The analysis of complete mitogenomes and of partial COI sequences of fifty-two Cacopsylla individuals showed a high homogeneity of genotypes over 15 years and among the different localities in the Czech Republic.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/QK21020395" target="_blank" >QK21020395: Produkce fytoplazem prostých školkařských výpěstků a jejich ochrana před reinfekcí</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agronomy

ISSN

2073-4395

e-ISSN

2073-4395

Svazek periodika

13

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

14

Strana od-do

2210

Kód UT WoS článku

001072879300001

EID výsledku v databázi Scopus

2-s2.0-85172667916