Acute and sub-acute oral toxicity assessment of the methanol root extract of Olax subscorpioidea Oliv. (Olacaceae) in mice and rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00578664" target="_blank" >RIV/61389030:_____/23:00578664 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/23:73622975

Výsledek na webu

<a href="https://doi.org/10.1016/j.sajb.2023.10.051" target="_blank" >https://doi.org/10.1016/j.sajb.2023.10.051</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.sajb.2023.10.051" target="_blank" >10.1016/j.sajb.2023.10.051</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Acute and sub-acute oral toxicity assessment of the methanol root extract of Olax subscorpioidea Oliv. (Olacaceae) in mice and rats

Popis výsledku v původním jazyce

Olax subscorpioidea Oliv. is an important medicinal plant included in African traditional anticancer medicines. Little information about its safety and toxicity appears in the literature. This study thus aimed at evaluating the safety of its methanol root extract orally administered in laboratory animals. Fourier Transform Infrared (FTIR) spectrophotometric analysis of O. subscorpioidea extract was conducted. For acute toxicity, methods within the Organization for Economic Co-operation and Development (OECD) guidelines were used, with slight modifications. A single female mouse was orally administered a dose of 2,000 mg/kg body weight of O. subscorpioidea methanol root extract and was monitored for at least 24 h for any signs of toxicity or death. An additional four mice (n = 4, 2/sex) were similarly treated. Another female mouse was orally administered 5,000 mg/kg body weight and observed for at least 24 h. Additional four mice were similarly treated. For sub-acute toxicity, the extract was administered daily to Wistar rats for 21 days at 250 mg/kg, 500 mg/kg, and 1,000 mg/kg. General behaviours changes, haematological, biochemical, and histological examinations on rats’ organs were performed. The FTIR spectrum showed no peaks in the 2220–2260 cm−1 region, suggesting that no cyanide groups were present in the extract. A single oral dose at both 2000 mg/kg and 5000 mg/kg did not induce any signs of toxicity or mortality, indicating an oral median lethal dose (LD50) >5000 mg/kg. When compared to the controls, dose-dependent changes were observed in the red blood cells, haemoglobin and haematocrit levels at 500 mg/kg and 1000 mg/kg (p<0.05). Histopathological examinations showed severe vascular congestion in the liver, and interstitial congestion and haemorrhage with moderate glomerular damage in the kidney. This study showed O. subscorpioidea root's methanol extract as non-toxic in acute administration, but with potential for toxicity in sub-acute oral administration.

Název v anglickém jazyce

Acute and sub-acute oral toxicity assessment of the methanol root extract of Olax subscorpioidea Oliv. (Olacaceae) in mice and rats

Popis výsledku anglicky

Olax subscorpioidea Oliv. is an important medicinal plant included in African traditional anticancer medicines. Little information about its safety and toxicity appears in the literature. This study thus aimed at evaluating the safety of its methanol root extract orally administered in laboratory animals. Fourier Transform Infrared (FTIR) spectrophotometric analysis of O. subscorpioidea extract was conducted. For acute toxicity, methods within the Organization for Economic Co-operation and Development (OECD) guidelines were used, with slight modifications. A single female mouse was orally administered a dose of 2,000 mg/kg body weight of O. subscorpioidea methanol root extract and was monitored for at least 24 h for any signs of toxicity or death. An additional four mice (n = 4, 2/sex) were similarly treated. Another female mouse was orally administered 5,000 mg/kg body weight and observed for at least 24 h. Additional four mice were similarly treated. For sub-acute toxicity, the extract was administered daily to Wistar rats for 21 days at 250 mg/kg, 500 mg/kg, and 1,000 mg/kg. General behaviours changes, haematological, biochemical, and histological examinations on rats’ organs were performed. The FTIR spectrum showed no peaks in the 2220–2260 cm−1 region, suggesting that no cyanide groups were present in the extract. A single oral dose at both 2000 mg/kg and 5000 mg/kg did not induce any signs of toxicity or mortality, indicating an oral median lethal dose (LD50) >5000 mg/kg. When compared to the controls, dose-dependent changes were observed in the red blood cells, haemoglobin and haematocrit levels at 500 mg/kg and 1000 mg/kg (p<0.05). Histopathological examinations showed severe vascular congestion in the liver, and interstitial congestion and haemorrhage with moderate glomerular damage in the kidney. This study showed O. subscorpioidea root's methanol extract as non-toxic in acute administration, but with potential for toxicity in sub-acute oral administration.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

South African Journal of Botany

ISSN

0254-6299

e-ISSN

1727-9321

Svazek periodika

163

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

157-164

Kód UT WoS článku

001110294300001

EID výsledku v databázi Scopus

2-s2.0-85174951185