Flow cytometric analysis and sorting of plant chromosomes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00579314" target="_blank" >RIV/61389030:_____/23:00579314 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s13237-023-00450-6" target="_blank" >https://doi.org/10.1007/s13237-023-00450-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13237-023-00450-6" target="_blank" >10.1007/s13237-023-00450-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flow cytometric analysis and sorting of plant chromosomes

Popis výsledku v původním jazyce

Flow cytogenetics is a high-throughput technique that classifies large populations of mitotic chromosomes according to their fluorescence and light scattering as they move in a single file rapidly in a narrow stream of liquid. Flow karyotyping can detect structural and numerical chromosome changes, and chromosomes purified by sorting have been used to discover the three-dimensional organization of DNA, characterize the proteome, and describe the organization of the perichromosomal layer at the nanometre level. However, the most frequent and important use of flow cytogenetics has been the isolation of different chromosomes. Chromosome sorting has made it possible to dissect nuclear genomes into small and defined parts, therefore allowing targeted, simplified, and more economical genomic analyses. The uses of flow-sorted chromosomes for genome complexity reduction and targeted analysis have expanded hand-in-hand with the progress of molecular biology and genomics techniques. These uses include the targeted development of DNA markers, the construction of chromosome-specific DNA libraries, physical mapping, draft genome sequencing, and gene cloning.

Název v anglickém jazyce

Flow cytometric analysis and sorting of plant chromosomes

Popis výsledku anglicky

Flow cytogenetics is a high-throughput technique that classifies large populations of mitotic chromosomes according to their fluorescence and light scattering as they move in a single file rapidly in a narrow stream of liquid. Flow karyotyping can detect structural and numerical chromosome changes, and chromosomes purified by sorting have been used to discover the three-dimensional organization of DNA, characterize the proteome, and describe the organization of the perichromosomal layer at the nanometre level. However, the most frequent and important use of flow cytogenetics has been the isolation of different chromosomes. Chromosome sorting has made it possible to dissect nuclear genomes into small and defined parts, therefore allowing targeted, simplified, and more economical genomic analyses. The uses of flow-sorted chromosomes for genome complexity reduction and targeted analysis have expanded hand-in-hand with the progress of molecular biology and genomics techniques. These uses include the targeted development of DNA markers, the construction of chromosome-specific DNA libraries, physical mapping, draft genome sequencing, and gene cloning.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NUCLEUS

ISSN

0029-568X

e-ISSN

—

Svazek periodika

66

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

IN - Indická republika

Počet stran výsledku

15

Strana od-do

355-369

Kód UT WoS článku

001101463300001

EID výsledku v databázi Scopus

2-s2.0-85176741896