Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00585625" target="_blank" >RIV/61389030:_____/24:00585625 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/24:73622044

Výsledek na webu

<a href="https://doi.org/10.1111/ppl.14144" target="_blank" >https://doi.org/10.1111/ppl.14144</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppl.14144" target="_blank" >10.1111/ppl.14144</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state

Popis výsledku v původním jazyce

Light is an essential environmental signal for plant development called photomorphogenesis. Here, we show that diethyl ether anaesthesia inhibits the de-etiolation process in barley (Hordeum vulgare) seedlings. Illuminated seedlings exposed to diethyl ether accumulated significantly less chlorophylls and chlorophyll-binding proteins, and exhibited reduced maximum quantum yield of photosystem II photochemistry (Fv/Fm). Although the direct effect of light necessary for the greening process, i.e. for the photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) catalysed by light-dependent protochlorophyllide oxidoreductase A (PORA), was not inhibited, the RNA-seq and qPCR analyses showed that light-induced expression of photosynthesis-associated nuclear genes (PhANGs) and genes encoding enzymes for chlorophyll biosynthesis were attenuated. On the other hand, transcription of chloroplast-encoded genes was not negatively affected by diethyl ether treatment during greening. Among the genes negatively regulated by light, PORA and PHYA were only slightly affected by diethyl ether. The effect of diethyl ether was fully reversible and, after its removal, the greening process was fully restored. Our data indicate that diethyl ether had two effects on greening: i) it inhibited the expression of PhANGs and chlorophyll biosynthesis-related genes irrespective of light conditions, ii) it blocked the light-induced expression of these genes and greening process of etiolated seedlings. Our study indicates that diethyl ether affects plastid biogenesis, which alters the orchestration of negative and positive regulators affecting phytochrome and/or retrograde signalling and does not allow expression of PhANGs. Thus, the plants are locked in an intermediate skoto-photomorphogenetic state in the light.

Název v anglickém jazyce

Diethyl ether anaesthesia inhibits de-etiolation of barley seedlings by locking them in intermediate skoto-photomorphogenetic state

Popis výsledku anglicky

Light is an essential environmental signal for plant development called photomorphogenesis. Here, we show that diethyl ether anaesthesia inhibits the de-etiolation process in barley (Hordeum vulgare) seedlings. Illuminated seedlings exposed to diethyl ether accumulated significantly less chlorophylls and chlorophyll-binding proteins, and exhibited reduced maximum quantum yield of photosystem II photochemistry (Fv/Fm). Although the direct effect of light necessary for the greening process, i.e. for the photoreduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) catalysed by light-dependent protochlorophyllide oxidoreductase A (PORA), was not inhibited, the RNA-seq and qPCR analyses showed that light-induced expression of photosynthesis-associated nuclear genes (PhANGs) and genes encoding enzymes for chlorophyll biosynthesis were attenuated. On the other hand, transcription of chloroplast-encoded genes was not negatively affected by diethyl ether treatment during greening. Among the genes negatively regulated by light, PORA and PHYA were only slightly affected by diethyl ether. The effect of diethyl ether was fully reversible and, after its removal, the greening process was fully restored. Our data indicate that diethyl ether had two effects on greening: i) it inhibited the expression of PhANGs and chlorophyll biosynthesis-related genes irrespective of light conditions, ii) it blocked the light-induced expression of these genes and greening process of etiolated seedlings. Our study indicates that diethyl ether affects plastid biogenesis, which alters the orchestration of negative and positive regulators affecting phytochrome and/or retrograde signalling and does not allow expression of PhANGs. Thus, the plants are locked in an intermediate skoto-photomorphogenetic state in the light.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA21-03593S" target="_blank" >GA21-03593S: Anestezie v rostlinách: signalizace a odpovědi</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiologia Plantarum

ISSN

0031-9317

e-ISSN

1399-3054

Svazek periodika

176

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

e14144

Kód UT WoS článku

001142148500001

EID výsledku v databázi Scopus

2-s2.0-85182481312