Light-Induced Changes in Phytohormone Levels of Developing Sunflower (Helianthus annuus L.) Seedlings

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00599612" target="_blank" >RIV/61389030:_____/24:00599612 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s00344-024-11321-y" target="_blank" >https://doi.org/10.1007/s00344-024-11321-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00344-024-11321-y" target="_blank" >10.1007/s00344-024-11321-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Light-Induced Changes in Phytohormone Levels of Developing Sunflower (Helianthus annuus L.) Seedlings

Popis výsledku v původním jazyce

Daily changes in the content of phytohormones accumulating in the hypocotyls of young sunflower seedlings that grow under a 14/10-h light-to-dark (LD) photoperiod were followed using LC-MS. The objective of the work was to investigate whether the presence of light and light transitions lead to visible changes visible in phytohormone levels on the sixth day after the onset of germination. The LC-MS technique used allowed the simultaneous detection of more than one hundred individual phytohormone derivatives, of which the daily accumulation patterns were determined for nearly fifty members. The daily changes in phytohormone levels followed specific patterns for each phytohormone. There were differences between the daytime and nighttime levels, most likely reflecting the effects of light on phytohormone metabolism. A significant difference was found between cytokinins (CKs) and all other phytohormones, so that CK group of phytohormones may be considered to have a separate role in hypocotyl elongation. Prolonged daytime (postponed dusk) resulted in a rapid disruption of rhythmic hypocotyl elongation and it triggered an acute light stress response that was evident in increased levels of a number of important phytohormones from all groups except CKs. However, this light stress, termed ARELD (Acute Response to Extended Light Duration), was not present in control plants grown under continuous light conditions. The data suggest that sunflower has means to adapt to the potentially stressful conditions of continuous light (LL). The qPCR-RT study of the isoforms of circadian clock-associated genes HaLHY, HaTOC1, HaELF3, and HaPIF3 revealed diurnal rhythms under both LD and LL conditions in which the peaks were synchronized and shifted toward the subjective dawn.

Název v anglickém jazyce

Light-Induced Changes in Phytohormone Levels of Developing Sunflower (Helianthus annuus L.) Seedlings

Popis výsledku anglicky

Daily changes in the content of phytohormones accumulating in the hypocotyls of young sunflower seedlings that grow under a 14/10-h light-to-dark (LD) photoperiod were followed using LC-MS. The objective of the work was to investigate whether the presence of light and light transitions lead to visible changes visible in phytohormone levels on the sixth day after the onset of germination. The LC-MS technique used allowed the simultaneous detection of more than one hundred individual phytohormone derivatives, of which the daily accumulation patterns were determined for nearly fifty members. The daily changes in phytohormone levels followed specific patterns for each phytohormone. There were differences between the daytime and nighttime levels, most likely reflecting the effects of light on phytohormone metabolism. A significant difference was found between cytokinins (CKs) and all other phytohormones, so that CK group of phytohormones may be considered to have a separate role in hypocotyl elongation. Prolonged daytime (postponed dusk) resulted in a rapid disruption of rhythmic hypocotyl elongation and it triggered an acute light stress response that was evident in increased levels of a number of important phytohormones from all groups except CKs. However, this light stress, termed ARELD (Acute Response to Extended Light Duration), was not present in control plants grown under continuous light conditions. The data suggest that sunflower has means to adapt to the potentially stressful conditions of continuous light (LL). The qPCR-RT study of the isoforms of circadian clock-associated genes HaLHY, HaTOC1, HaELF3, and HaPIF3 revealed diurnal rhythms under both LD and LL conditions in which the peaks were synchronized and shifted toward the subjective dawn.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EH22_008%2F0004581" target="_blank" >EH22_008/0004581: Nové poznatky pro plodiny nové generace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Plant Growth Regulation

ISSN

0721-7595

e-ISSN

1435-8107

Svazek periodika

43

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

15

Strana od-do

3528-3542

Kód UT WoS článku

001214082700001

EID výsledku v databázi Scopus

2-s2.0-85192016822