The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00599652" target="_blank" >RIV/61389030:_____/24:00599652 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652079:_____/24:00599652 RIV/61989592:15310/24:73627241

Výsledek na webu

<a href="https://doi.org/10.1016/j.cj.2024.07.003" target="_blank" >https://doi.org/10.1016/j.cj.2024.07.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.cj.2024.07.003" target="_blank" >10.1016/j.cj.2024.07.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

Popis výsledku v původním jazyce

Plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental factors. Haploids/doubled haploids (DHs) derived from in vitro-cultured microspores are widely used in plant breeding and genetic engineering. However, the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined. Here, RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis (ME) in barley cultivars Golden Promise and Igri, which exhibit contrasting responsiveness to microspore-derived plant formation. Our experiments revealed fundamental regulatory networks, specific groups of genes, and transcription factor (TF) families potentially regulating the developmental switch. We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME. Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.

Název v anglickém jazyce

The transcriptional landscape of the developmental switch from regular pollen maturation towards microspore-derived plant regeneration in barley

Popis výsledku anglicky

Plant formation from in vitro-cultivated microspores involves a complex network of internal and environmental factors. Haploids/doubled haploids (DHs) derived from in vitro-cultured microspores are widely used in plant breeding and genetic engineering. However, the mechanism underlying the developmental switch from regular pollen maturation towards microspore-derived plant regeneration remains poorly defined. Here, RNA-sequencing was employed to elucidate the transcriptional landscapes of four early stages of microspore embryogenesis (ME) in barley cultivars Golden Promise and Igri, which exhibit contrasting responsiveness to microspore-derived plant formation. Our experiments revealed fundamental regulatory networks, specific groups of genes, and transcription factor (TF) families potentially regulating the developmental switch. We identified a set of candidate genes crucial for genotype-dependent responsiveness/recalcitrance to ME. Our high-resolution temporal transcriptome atlas provides an important resource for future functional studies on the genetic control of microspore developmental transition.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Crop Journal

ISSN

2095-5421

e-ISSN

2214-5141

Svazek periodika

12

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CN - Čínská lidová republika

Počet stran výsledku

17

Strana od-do

1064-1080

Kód UT WoS článku

001318229900001

EID výsledku v databázi Scopus

2-s2.0-85200572711