Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00600473" target="_blank" >RIV/61389030:_____/24:00600473 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/24:73628743

Výsledek na webu

<a href="https://doi.org/10.1126/science.adj4591" target="_blank" >https://doi.org/10.1126/science.adj4591</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1126/science.adj4591" target="_blank" >10.1126/science.adj4591</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export

Popis výsledku v původním jazyce

Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.

Název v anglickém jazyce

Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export

Popis výsledku anglicky

Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science

ISSN

0036-8075

e-ISSN

1095-9203

Svazek periodika

383

Číslo periodika v rámci svazku

6689

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

eadj4591

Kód UT WoS článku

001252955200028

EID výsledku v databázi Scopus

2-s2.0-85188561130