Multiple mutations in polyketide synthase led to disruption of Psittacofulvin production across diverse parrot species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00616905" target="_blank" >RIV/61389030:_____/25:00616905 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1038/s42003-025-07537-7" target="_blank" >https://doi.org/10.1038/s42003-025-07537-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s42003-025-07537-7" target="_blank" >10.1038/s42003-025-07537-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiple mutations in polyketide synthase led to disruption of Psittacofulvin production across diverse parrot species

Popis výsledku v původním jazyce

Polyketide synthases (PKSs) are crucial multidomain enzymes in diverse natural product biosynthesis. Parrots use a type I PKS to produce a unique pigment called psittacofulvin in their feathers. In domesticated budgerigars and lovebirds, the same amino acid substitution (R644W) within malonyl/acetyltransferase (MAT) domain of this enzyme has been shown to cause the blue phenotype with no psittacofulvin pigmentation, proposing a strong evolutionary constraint on the mechanism. Here, we identified seven previously unreported variants in PKS associated with defective psittacofulvin production in four diverse species, including three nonsense mutations. Intriguingly, three of the remaining nonsynonymous substitutions reside within the ketoacyl synthase (KS) domain, whereas one at MAT domain. The heterologous expression of these PKS variants in yeast confirmed complete or partial loss of psittacofulvin production. These findings establish PKS as a functionally conserved key-enzyme determining psittacofulvin-based hues among diverse parrots, highlighting multiple conserved domains essential for the PKS function.

Název v anglickém jazyce

Multiple mutations in polyketide synthase led to disruption of Psittacofulvin production across diverse parrot species

Popis výsledku anglicky

Polyketide synthases (PKSs) are crucial multidomain enzymes in diverse natural product biosynthesis. Parrots use a type I PKS to produce a unique pigment called psittacofulvin in their feathers. In domesticated budgerigars and lovebirds, the same amino acid substitution (R644W) within malonyl/acetyltransferase (MAT) domain of this enzyme has been shown to cause the blue phenotype with no psittacofulvin pigmentation, proposing a strong evolutionary constraint on the mechanism. Here, we identified seven previously unreported variants in PKS associated with defective psittacofulvin production in four diverse species, including three nonsense mutations. Intriguingly, three of the remaining nonsynonymous substitutions reside within the ketoacyl synthase (KS) domain, whereas one at MAT domain. The heterologous expression of these PKS variants in yeast confirmed complete or partial loss of psittacofulvin production. These findings establish PKS as a functionally conserved key-enzyme determining psittacofulvin-based hues among diverse parrots, highlighting multiple conserved domains essential for the PKS function.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Communications Biology

ISSN

2399-3642

e-ISSN

2399-3642

Svazek periodika

8

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

69

Kód UT WoS článku

001400116100003

EID výsledku v databázi Scopus

2-s2.0-85216067745